从茴鱼中提取透明质酸和软骨素/硫酸皮肤素的特性研究。

Characterisation of hyaluronic acid and chondroitin/dermatan sulfate from the lumpsucker fish, C. lumpus.

机构信息

EaStCHEM School of Chemistry, Joseph Black Building, The King's Buildings, Edinburgh EH9 3JJ, UK.

GlycoMar Ltd, European Centre for Marine Biotechnology, Oban, Scotland PA37 1QA, UK.

出版信息

Carbohydr Polym. 2014 Jun 15;106:25-33. doi: 10.1016/j.carbpol.2014.01.090. Epub 2014 Feb 5.

DOI:10.1016/j.carbpol.2014.01.090

PMID:24721047

Abstract

The lumpsucker, Cyclopterus lumpus, a cottoid teleost fish found in the cold waters of the North Atlantic, and North Pacific, was identified as a possible source of GAGs. The GAGs present in the C. lumpus dorsal hump and body wall tissue were isolated and purified. Two fractions were analysed by NMR and their GAG structures determined as hyaluronic acid and CS/DS chains. The latter fraction contained GlcA (65% of the total uronic acids) and IdoA (the remaining 35%). All uronic acid residues were unsulfated, whilst 86% of the GalNAc was 4-sulfated and 14% was 6-sulfated. The presence of GlcA-GalNAc4S, IdoA-GalNAc4S and GlcA-GalNAc6S disaccharide fragments was confirmed. The isolated GAGs obtained from each tissue were biochemically characterised. The lumpsucker offers a high yield source of GAGs, which compares favourably with other sources such as shark cartilage.

摘要

圆鳍鱼，一种产于北大西洋和北太平洋冷水域的 Cottid 硬骨鱼，被确定为 GAGs 的潜在来源。从 C. lumpus 的背鳍瘤和体壁组织中分离和纯化了 GAGs。通过 NMR 对两种馏分进行了分析，并确定了它们的 GAG 结构为透明质酸和 CS/DS 链。后一种馏分含有 GlcA（总糖醛酸的 65%）和 IdoA（其余的 35%）。所有糖醛酸残基均未硫酸化，而 86%的 GalNAc 为 4-硫酸化，14%为 6-硫酸化。证实存在 GlcA-GalNAc4S、IdoA-GalNAc4S 和 GlcA-GalNAc6S 二糖片段。从每种组织中分离得到的 GAGs 在生化上进行了表征。圆鳍鱼是 GAGs 的高产量来源，与鲨鱼软骨等其他来源相比具有优势。