Changchun Center of Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 5625 Renmin Street, Changchun 130022, P, R, China.
Clin Proteomics. 2014 Apr 10;11(1):14. doi: 10.1186/1559-0275-11-14.
Glycosylation is one of the most important posttranslational modifications of proteins and plays essential roles in various biological processes. Aberration in the glycan moieties of glycoproteins is associated with many diseases. It is especially critical to develop the rapid and sensitive methods for analysis of aberrant glycoproteins associated with diseases. Mass spectrometry (MS) has become a powerful tool for glycoprotein analysis. Especially, tandem mass spectrometry can provide highly informative fragments for structural identification of glycoproteins. This review provides an overview of the development of MS technologies and their applications in identification of abnormal glycoproteins and glycans in human serum to screen cancer biomarkers in recent years.
糖基化是蛋白质最重要的翻译后修饰之一，在各种生物过程中起着至关重要的作用。糖蛋白中聚糖部分的异常与许多疾病有关。因此，开发用于分析与疾病相关的异常糖蛋白的快速、灵敏的方法尤为关键。质谱（MS）已成为糖蛋白分析的有力工具。特别是串联质谱可以为糖蛋白的结构鉴定提供高度信息丰富的片段。本文综述了近年来 MS 技术的发展及其在鉴定人血清中异常糖蛋白和聚糖以筛选癌症生物标志物方面的应用。
