基于质谱的糖蛋白分析及其在癌症生物标志物发现中的临床应用。
Mass spectrometry-based analysis of glycoproteins and its clinical applications in cancer biomarker discovery.
机构信息
Changchun Center of Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 5625 Renmin Street, Changchun 130022, P, R, China.
出版信息
Clin Proteomics. 2014 Apr 10;11(1):14. doi: 10.1186/1559-0275-11-14.
Abstract
Glycosylation is one of the most important posttranslational modifications of proteins and plays essential roles in various biological processes. Aberration in the glycan moieties of glycoproteins is associated with many diseases. It is especially critical to develop the rapid and sensitive methods for analysis of aberrant glycoproteins associated with diseases. Mass spectrometry (MS) has become a powerful tool for glycoprotein analysis. Especially, tandem mass spectrometry can provide highly informative fragments for structural identification of glycoproteins. This review provides an overview of the development of MS technologies and their applications in identification of abnormal glycoproteins and glycans in human serum to screen cancer biomarkers in recent years.
摘要
糖基化是蛋白质最重要的翻译后修饰之一，在各种生物过程中起着至关重要的作用。糖蛋白中聚糖部分的异常与许多疾病有关。因此，开发用于分析与疾病相关的异常糖蛋白的快速、灵敏的方法尤为关键。质谱（MS）已成为糖蛋白分析的有力工具。特别是串联质谱可以为糖蛋白的结构鉴定提供高度信息丰富的片段。本文综述了近年来 MS 技术的发展及其在鉴定人血清中异常糖蛋白和聚糖以筛选癌症生物标志物方面的应用。
相似文献
1
Mass spectrometry-based analysis of glycoproteins and its clinical applications in cancer biomarker discovery.基于质谱的糖蛋白分析及其在癌症生物标志物发现中的临床应用。
Clin Proteomics. 2014 Apr 10;11(1):14. doi: 10.1186/1559-0275-11-14.
2
Recent advances in mass spectrometric analysis of glycoproteins.糖蛋白质谱分析的最新进展
Electrophoresis. 2017 Jan;38(1):162-189. doi: 10.1002/elps.201600357. Epub 2016 Dec 15.
3
Mass Spectrometry-Based Chemical and Enzymatic Methods for Global Analysis of Protein Glycosylation.基于质谱的蛋白质糖基化全局分析的化学和酶学方法。
Acc Chem Res. 2018 Aug 21;51(8):1796-1806. doi: 10.1021/acs.accounts.8b00200. Epub 2018 Jul 16.
4
Quantitative O-glycomics based on improvement of the one-pot method for nonreductive O-glycan release and simultaneous stable isotope labeling with 1-(d/d)phenyl-3-methyl-5-pyrazolone followed by mass spectrometric analysis.基于改进一锅法的定量O-糖组学，该方法用于非还原O-聚糖释放及同时用1-(氘/氘代)phenyl-3-methyl-5-pyrazolone进行稳定同位素标记，随后进行质谱分析。
J Proteomics. 2017 Jan 6;150:18-30. doi: 10.1016/j.jprot.2016.08.012. Epub 2016 Aug 29.
5
Reductive chemical release of N-glycans as 1-amino-alditols and subsequent 9-fluorenylmethyloxycarbonyl labeling for MS and LC/MS analysis.通过还原化学方法释放 N-糖链为 1-氨基-醛糖醇，然后进行 9-芴甲氧羰酰基（FMOC）标记，用于 MS 和 LC/MS 分析。
J Proteomics. 2018 Sep 15;187:47-58. doi: 10.1016/j.jprot.2018.06.002. Epub 2018 Jun 6.
6
High-throughput and high-sensitivity N-Glycan profiling: A platform for biopharmaceutical development and disease biomarker discovery.高通量和高灵敏度 N-糖链分析：用于生物制药开发和疾病生物标志物发现的平台。
Anal Biochem. 2021 Jun 15;623:114205. doi: 10.1016/j.ab.2021.114205. Epub 2021 Apr 20.
7
Parallel data acquisition of in-source fragmented glycopeptides to sequence the glycosylation sites of proteins.源内片段化糖肽的并行数据采集以对蛋白质的糖基化位点进行测序。
Electrophoresis. 2016 Jun;37(11):1420-30. doi: 10.1002/elps.201500562. Epub 2016 Apr 9.
8
Mass Spectrometric Analysis of N-Glycoforms of Soybean Allergenic Glycoproteins Separated by SDS-PAGE.通过SDS-PAGE分离的大豆致敏糖蛋白N-糖型的质谱分析
J Agric Food Chem. 2016 Oct 5;64(39):7367-7376. doi: 10.1021/acs.jafc.6b02773. Epub 2016 Sep 21.
9
Capillary electrophoresis-electrospray ionization mass spectrometry for rapid and sensitive N-glycan analysis of glycoproteins as 9-fluorenylmethyl derivatives.毛细管电泳-电喷雾电离质谱法用于快速灵敏地分析作为9-芴基甲基衍生物的糖蛋白的N-聚糖。
Glycobiology. 2009 Feb;19(2):135-43. doi: 10.1093/glycob/cwn115. Epub 2008 Oct 25.
10
Antibody Panel Based N-Glycan Imaging for N-Glycoprotein Biomarker Discovery.基于抗体芯片的N-聚糖成像用于发现N-糖蛋白生物标志物
Curr Protoc Protein Sci. 2019 Dec;98(1):e99. doi: 10.1002/cpps.99.
引用本文的文献
1
Enhanced N-Glycan Profiling of Therapeutic Monoclonal Antibodies through the Application of Upper-Hinge Middle-Up Level LC-HRMS Analysis.通过应用上铰链区中上游水平液相色谱-高分辨质谱分析增强治疗性单克隆抗体的N-聚糖谱分析
Antibodies (Basel). 2024 Aug 6;13(3):66. doi: 10.3390/antib13030066.
2
Quantitative clinical glycomics strategies: A guide for selecting the best analysis approach.定量临床糖组学策略：选择最佳分析方法的指南。
Mass Spectrom Rev. 2022 Nov;41(6):901-921. doi: 10.1002/mas.21688. Epub 2021 Feb 10.
3
Leveraging glycomics data in glycoprotein 3D structure validation with Privateer.利用糖组学数据通过Privateer进行糖蛋白3D结构验证。
Beilstein J Org Chem. 2020 Oct 9;16:2523-2533. doi: 10.3762/bjoc.16.204. eCollection 2020.
4
Comparison of Different HILIC Stationary Phases in the Separation of Hemopexin and Immunoglobulin G Glycopeptides and Their Isomers.比较不同亲水作用色谱固定相在分离血红素结合蛋白和免疫球蛋白 G 糖肽及其异构体中的应用。
Molecules. 2020 Oct 13;25(20):4655. doi: 10.3390/molecules25204655.
5
Rapid Glycopeptide Enrichment Using Cellulose Hydrophilic Interaction/Reversed-Phase StageTips.使用纤维素亲水相互作用/反相阶段Tips快速富集糖肽
Mass Spectrom (Tokyo). 2017;6(1):A0061. doi: 10.5702/massspectrometry.A0061. Epub 2017 Aug 23.
6
Discovery of Non-invasive Glycan Biomarkers for Detection and Surveillance of Gastric Cancer.用于胃癌检测和监测的非侵入性聚糖生物标志物的发现
J Cancer. 2017 Jul 3;8(10):1908-1916. doi: 10.7150/jca.17900. eCollection 2017.
7
Direct analysis of aberrant glycosylation on haptoglobin in patients with gastric cancer.胃癌患者触珠蛋白异常糖基化的直接分析。
Oncotarget. 2017 Feb 14;8(7):11094-11104. doi: 10.18632/oncotarget.14362.
8
A lectin-based approach to detecting carcinogenesis in breast tissue.一种基于凝集素的检测乳腺组织癌变的方法。
Oncol Lett. 2016 Jun;11(6):3889-3895. doi: 10.3892/ol.2016.4456. Epub 2016 Apr 18.
9
Direct analysis of hCGβcf glycosylation in normal and aberrant pregnancy by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.通过基质辅助激光解吸/电离飞行时间质谱法直接分析正常妊娠和异常妊娠中的人绒毛膜促性腺激素β核心片段糖基化情况。
Int J Mol Sci. 2014 Jun 5;15(6):10067-82. doi: 10.3390/ijms150610067.
本文引用的文献
1
High-field asymmetric-waveform ion mobility spectrometry and electron detachment dissociation of isobaric mixtures of glycosaminoglycans.高场非对称波形离子迁移谱法与糖胺聚糖等压混合物的电子脱附解离
J Am Soc Mass Spectrom. 2014 Feb;25(2):258-68. doi: 10.1007/s13361-013-0771-1. Epub 2013 Nov 20.
2
Quantitative analysis of aberrant protein glycosylation in liver cancer plasma by AAL-enrichment and MRM mass spectrometry.通过 AAL 富集和 MRM 质谱分析肝癌血浆中异常蛋白糖基化的定量分析。
Analyst. 2013 Nov 7;138(21):6454-62. doi: 10.1039/c3an01126g.
3
Mass spectrometry of glycans.聚糖的质谱分析。
Biochemistry (Mosc). 2013 Jul;78(7):710-20. doi: 10.1134/S0006297913070031.
4
Glycoproteomic analysis of bronchoalveolar lavage (BAL) fluid identifies tumor-associated glycoproteins from lung adenocarcinoma.支气管肺泡灌洗液（BAL）的糖蛋白质组学分析鉴定出肺腺癌相关的糖蛋白。
J Proteome Res. 2013 Aug 2;12(8):3689-96. doi: 10.1021/pr400274w. Epub 2013 Jul 11.
5
Change in IgG1 Fc N-linked glycosylation in human lung cancer: age- and sex-related diagnostic potential.人肺癌 IgG1 Fc 糖基化的变化：与年龄和性别相关的诊断潜力。
Electrophoresis. 2013 Aug;34(16):2407-16. doi: 10.1002/elps.201200455. Epub 2013 Jul 26.
6
Altered expression of sialylated glycoproteins in ovarian cancer sera using lectin-based ELISA assay and quantitative glycoproteomics analysis.基于凝集素的 ELISA 检测和定量糖蛋白质组学分析检测卵巢癌血清中唾液酸化糖蛋白的表达变化。
J Proteome Res. 2013 Jul 5;12(7):3342-52. doi: 10.1021/pr400169n. Epub 2013 Jun 17.
7
High-sensitivity analytical approaches for the structural characterization of glycoproteins.用于糖蛋白结构表征的高灵敏度分析方法。
Chem Rev. 2013 Apr 10;113(4):2668-732. doi: 10.1021/cr3003714. Epub 2013 Mar 27.
8
Identification of new apolipoprotein-CIII glycoforms with ultrahigh resolution MALDI-FTICR mass spectrometry of human sera.利用 MALDI-FTICR 质谱超高分辨率对人血清中的新型载脂蛋白 CIII 糖型进行鉴定。
J Proteome Res. 2013 May 3;12(5):2260-8. doi: 10.1021/pr400136p. Epub 2013 Apr 5.
9
Chip-based nLC-TOF-MS is a highly stable technology for large-scale high-throughput analyses.基于芯片的纳升液相色谱-飞行时间质谱是一种用于大规模高通量分析的高稳定性技术。
Anal Bioanal Chem. 2013 May;405(14):4953-8. doi: 10.1007/s00216-013-6908-z. Epub 2013 Mar 23.
10
Comparative glycomic profiling of isotopically permethylated N-glycans by liquid chromatography/electrospray ionization mass spectrometry.利用液相色谱/电喷雾电离质谱技术对同位素全甲基化 N-糖进行糖组学比较分析。
Rapid Commun Mass Spectrom. 2013 Apr 30;27(8):865-77. doi: 10.1002/rcm.6512.
Zotero 插件