Center for Biomedical Mass Spectrometry, Boston University School of Medicine, Boston, MA 02118, USA.
Biochemistry (Mosc). 2013 Jul;78(7):710-20. doi: 10.1134/S0006297913070031.
Powerful new strategies based on mass spectrometry are revolutionizing the structural analysis and profiling of glycans and glycoconjugates. We survey here the major biosynthetic pathways that underlie the biological diversity in glycobiology, with emphasis on glycoproteins, and the approaches that can be used to address the resulting heterogeneity. Included among these are derivatizations, on- and off-line chromatography, electrospray and matrix-assisted laser desorption/ionization, and a variety of dissociation methods, the recently introduced electron-based techniques being of particular interest.
基于质谱的强大新策略正在彻底改变聚糖和糖缀合物的结构分析和分析。在这里，我们调查了糖生物学中生物多样性的主要生物合成途径，重点是糖蛋白，以及可用于解决由此产生的异质性的方法。其中包括衍生化、在线和离线色谱、电喷雾和基质辅助激光解吸/电离，以及各种解离方法，最近引入的基于电子的技术尤其引人注目。
