Schlemper Thiago Roberto, Stürmer Sidney Luiz
Programa de Pós-Graduação em Engenharia Ambiental, Universidade Regional de Blumenau (FURB), Rua São Paulo 3250, 89030-000, Blumenau, SC, Brazil.
Mycorrhiza. 2014 Nov;24(8):571-80. doi: 10.1007/s00572-014-0576-5. Epub 2014 Apr 12.
The present study evaluated the efficiency of lignocellulosic agrowastes produced in Brazil as substrates for production of on farm AMF inoculum and tested different diluents and inoculation techniques. In a first experiment, Sorghum bicolor seedlings were colonized with Rhizophagus clarus or Claroideoglomus etunicatus and transplanted to 20 L bags containing sugarcane bagasse (SC), king palm leaf sheets (KP), or barley hulls (BH) mixed (1:1:1 or 2:1:1, v/v/v) with sand and rice shell. SC and KP were conducive for production of spores and infectious propagules. A number of infectious propagules obtained were greater than with BH and ranged from 233-350 propagules cm(-3) for both isolates in SC and KP at the1:1:1 mix dilution. Number of spores of both fungi was affected mainly by the SC agrowaste, and spore densities were significantly higher compared to KP and BH. In a second experiment, SC was mixed with soil or sand and inoculation consisted of transplanting colonized seedlings or adding soil inoculum. Number of propagules tended to differ for each fungus according to the inoculation technique or diluent. It is concluded from the data that SC and KP are suitable agrowastes to be incorporated in substrates for producing AMF inoculum using the on farm method.
本研究评估了巴西生产的木质纤维素农业废弃物作为农场丛枝菌根真菌（AMF）接种剂生产基质的效率，并测试了不同的稀释剂和接种技术。在第一个实验中，用明球囊霉或埃氏无梗囊霉对双色高粱幼苗进行定殖，然后将其移植到装有甘蔗渣（SC）、王棕叶（KP）或大麦壳（BH）与沙子和稻壳混合（体积比1:1:1或2:1:1）的20升袋子中。SC和KP有利于孢子和感染性繁殖体的生产。在1:1:1混合稀释的SC和KP中，两种分离物获得的感染性繁殖体数量均大于BH，范围为233 - 350个繁殖体/cm³。两种真菌的孢子数量主要受SC农业废弃物的影响，与KP和BH相比，孢子密度显著更高。在第二个实验中，将SC与土壤或沙子混合，接种方式包括移植定殖的幼苗或添加土壤接种物。根据接种技术或稀释剂的不同，每种真菌的繁殖体数量往往有所不同。从数据得出结论，SC和KP是适合采用农场方法纳入生产AMF接种剂基质的农业废弃物。
