US Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center, Wyndmoor, PA 19038, USA.
Bioresour Technol. 2010 Apr;101(7):2326-30. doi: 10.1016/j.biortech.2009.11.071. Epub 2009 Dec 23.
On-farm production of arbuscular mycorrhizal [AM] fungus inoculum can be employed to make the benefits of the symbiosis more available to vegetable farmers. Experiments were conducted to modify an existing method for the production of inoculum in temperate climates to make it more readily adoptable by farmers. Perlite, vermiculite, and peat based potting media were tested as diluents of yard clippings compost for the media in which the inoculum was produced using bahiagrass (Paspalum notatum Flugge) as host plant. All produced satisfactory concentrations of AM fungus propagules, though vermiculite proved to be better than potting media (89 vs. 25 propagules cm(-3), respectively). Two methods were tested for the growth of AM fungi indigenous to the farm: (1) adding field soil into the vermiculite and compost mixture and (2) pre-colonizing the bahiagrass seedlings in media inoculated with field soil prior to transplant into that mixture. Adding 100 cm(3) of field soil to the compost and vermiculite produced 465 compared to 137 propagules cm(-3) for the pre-colonization method. The greater flexibility these modifications give will make it easier for farmers to produce inoculum of AM fungi on-the-farm.
在农场生产丛枝菌根（AM）真菌接种体可以使蔬菜种植者更广泛地受益于这种共生关系。本研究旨在改进现有的在温带气候下生产接种体的方法，使其更易于被农民采用。我们以雀稗（Paspalum notatum Flugge）为宿主植物，以珍珠岩、蛭石和泥炭为盆栽基质，测试了这些基质作为草坪草屑堆肥稀释剂的效果。所有的基质都产生了令人满意的丛枝菌根真菌繁殖体浓度，尽管蛭石的效果优于盆栽基质（分别为 89 和 25 个繁殖体 cm(-3)）。我们还测试了两种适用于农场内生丛枝菌根真菌的生长方法：（1）将田间土壤添加到珍珠岩和堆肥混合物中；（2）在将 bahiagrass 幼苗移植到含有田间土壤的混合物之前，预先用接种了田间土壤的培养基对幼苗进行接种。将 100 cm(3)的田间土壤添加到堆肥和珍珠岩中，产生了 465 个繁殖体 cm(-3)，而预先接种方法的繁殖体浓度为 137 个 cm(-3)。这些改进提供了更大的灵活性，使农民更容易在农场生产丛枝菌根真菌接种体。
