非洲绿猴作为使用Campath-1加补体进行骨髓T细胞清除体外研究的可靠模型。
Cercopithecus aethiops monkey as a reliable model for in vitro study of T cell depletion of bone marrow with Campath-1 plus complement.
作者信息
Phan D T, Benczur M, Gidáli J, Fehér I, Harsányi V, Nemes L, Petrányi G, Hollán S R
机构信息
National Institute of Haematology and Blood Transfusion, Budapest, Hungary.
出版信息
Folia Haematol Int Mag Klin Morphol Blutforsch. 1989;116(1):97-106.
T-lymphocyte markers of peripheral blood mononuclear cells (PBMCs) and bone marrow mononuclear cells (BMMCs) of C. aethiops monkeys were studied by using anti-human monoclonal antibodies. The results show that C. aethiops T lymphocytes express surface markers which react specifically with anti-human MoAbs including CD3, CD4, CD8, CD2. However, very few CD3-positive cells were found, in contrast to the abundance in CD8+ cells. There is a high conservation of receptors forming E rosettes with AET-treated SRBCs, and antigens reacting with the anti-human T and B cell monoclonal antibody (Campath-1). The present findings indicate that C. aethiops can be used as a new experimental model for studies on T-cell depletion from bone marrow with Campath-1 MoAb + rabbit C.
使用抗人单克隆抗体研究了埃塞俄比亚长尾猴外周血单个核细胞（PBMC）和骨髓单个核细胞（BMMC）的T淋巴细胞标志物。结果显示，埃塞俄比亚长尾猴T淋巴细胞表达与抗人单克隆抗体特异性反应的表面标志物，包括CD3、CD4、CD8、CD2。然而，与CD8 +细胞的丰富数量相比，发现CD3阳性细胞很少。与经AET处理的SRBC形成E花环的受体以及与抗人T和B细胞单克隆抗体（Campath-1）反应的抗原具有高度保守性。目前的研究结果表明，埃塞俄比亚长尾猴可作为使用Campath-1单克隆抗体+兔补体从骨髓中清除T细胞研究的新实验模型。
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