广泛对氯喹敏感的恶性疟原虫在马拉维重现。
Return of widespread chloroquine-sensitive Plasmodium falciparum to Malawi.
作者信息
Frosch Anne E P, Laufer Miriam K, Mathanga Don P, Takala-Harrison Shannon, Skarbinski Jacek, Claassen Cassidy W, Dzinjalamala Fraction K, Plowe Christopher V
机构信息
Howard Hughes Medical Institute/Center for Vaccine Development, University of Maryland School of Medicine, Baltimore Department of Medicine, University of Minnesota, Minneapolis.
Howard Hughes Medical Institute/Center for Vaccine Development, University of Maryland School of Medicine, Baltimore.
出版信息
J Infect Dis. 2014 Oct 1;210(7):1110-4. doi: 10.1093/infdis/jiu216. Epub 2014 Apr 9.
Abstract
BACKGROUND
The return of chloroquine-sensitive Plasmodium falciparum to the limited area of Blantyre, Malawi, has been well demonstrated in several studies.
METHODS
To characterize chloroquine susceptibility over a wide geographic area, infants and children aged 6-59 months were selected using 2-stage cluster sampling in 8 Malawian districts. Pyrosequencing of the pfcrt gene codon 76 region was performed for children with asexual parasitemia.
RESULTS
Of 7145 children, 1150 had microscopic asexual parasitemia, and sequencing was performed in 685, of whom 1 had a chloroquine-resistant genotype.
CONCLUSIONS
Systematic countrywide sampling demonstrates that the chloroquine pfcrt genotype has reached near-fixation, raising the possibility of reintroducing chloroquine for malaria prevention and treatment.
摘要
背景
多项研究已充分证明，对氯喹敏感的恶性疟原虫已重返马拉维布兰太尔的有限区域。
方法
为了在广泛的地理区域内表征氯喹敏感性，在马拉维的8个地区采用两阶段整群抽样法选取了6至59个月大的婴儿和儿童。对患有无性疟原虫血症的儿童进行pfcrt基因第76位密码子区域的焦磷酸测序。
结果
在7145名儿童中，1150名有镜检无性疟原虫血症，对其中685名进行了测序，其中1名具有氯喹抗性基因型。
结论
全国范围内的系统抽样表明，氯喹pfcrt基因型已接近固定，这增加了重新引入氯喹用于疟疾预防和治疗的可能性。
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