热休克可诱导培养细胞中的蛋白质酪氨酸磷酸化。
Heat shock induces protein tyrosine phosphorylation in cultured cells.
作者信息
Maher P A, Pasquale E B
机构信息
Biology Department, University of California, San Diego, La Jolla 92093.
出版信息
J Cell Biol. 1989 Jun;108(6):2029-35. doi: 10.1083/jcb.108.6.2029.
Abstract
We examined the effect of heat shock on protein tyrosine phosphorylation in cultured animal cells using antiphosphotyrosine antibodies in immunoblotting and immunofluorescence microscopy experiments. Heat shock significantly elevated the level of phosphotyrosine in proteins in most of the cultured cells examined, including fibroblasts, epithelial cells, nerve cells, and muscle cells, but not in Rous sarcoma virus-transformed fibroblasts. The increase in protein tyrosine phosphorylation induced by heat shock occurred in proteins with a wide range of molecular masses and was dependent on the temperature and duration of the heat shock.
摘要
我们在免疫印迹和免疫荧光显微镜实验中使用抗磷酸酪氨酸抗体，研究了热休克对培养的动物细胞中蛋白质酪氨酸磷酸化的影响。热休克显著提高了大多数被检测的培养细胞中蛋白质的磷酸酪氨酸水平，这些细胞包括成纤维细胞、上皮细胞、神经细胞和肌肉细胞，但在劳氏肉瘤病毒转化的成纤维细胞中未出现这种情况。热休克诱导的蛋白质酪氨酸磷酸化增加发生在分子量范围广泛的蛋白质中，并且取决于热休克的温度和持续时间。
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