Torikoshi Aiko, Namera Akira, Arima Yousuke, Toubou Hirokazu, Tajima Takashi, Shiraishi Hiroaki, Nagao Masataka
Chudoku Kenkyu. 2014 Mar;27(1):33-8.
Immunoassays are useful methods for the determination of regulated drugs in clinical and forensic laboratories. Although the Instant-View M-1 (IV M-1) immunoassay kit is frequently used to screen drugs in laboratories in Japan, basic information about the IV M-1 such as its specificity and reactivity is not available. In this study, we determined the specificity and cross-reactivity of IV M-1 for the detection of benzodiazepine-related drugs and their metabolites in urine. The IV M-1 could detect triazolobenzodiazepines such as triazolam in urine at concentrations > or = 300 ng/mL. However, thienodiazepines such as etizolam could not be detected because of lack of cross reactivity. A correlation was observed between the structure of the metabolites and the reactivity of the kit; 4-hydroxy metabolites of alprazolam and triazolam were detectable, whereas a-hydroxy metabolites were not. Furthermore, 7-amino metabolites such as nitrazepam could not be detected at any concentration, including high concentrations. The specificity and reactivity of various kits used for detection of drugs in urine are different. Therefore, it is necessary to consider the basic features of the kit used while assessing the results obtained.
免疫测定法是临床和法医实验室中测定管制药物的有用方法。尽管即时视M-1（IV M-1）免疫测定试剂盒在日本的实验室中经常用于药物筛查，但关于IV M-1的基本信息，如其特异性和反应性尚不可得。在本研究中，我们测定了IV M-1在检测尿液中苯二氮䓬类相关药物及其代谢物时的特异性和交叉反应性。IV M-1能够检测尿液中浓度大于或等于300 ng/mL的三唑苯二氮䓬类药物，如三唑仑。然而，由于缺乏交叉反应性，噻吩二氮䓬类药物如依替唑仑无法被检测到。观察到代谢物的结构与试剂盒的反应性之间存在相关性；阿普唑仑和三唑仑的4-羟基代谢物可被检测到，而α-羟基代谢物则不能。此外，硝西泮等7-氨基代谢物在任何浓度下，包括高浓度时，均无法被检测到。用于检测尿液中药物的各种试剂盒的特异性和反应性各不相同。因此，在评估所得结果时，有必要考虑所使用试剂盒的基本特征。
