Paliard X, Yssel H, Blanchard D, Waitz J A, deVries J E, Spits H
UNICET Laboratories for Immunological Research, Dardilly, France.
J Immunol. 1989 Jul 15;143(2):452-7.
IL-4 has been shown to act as a growth factor for human T cells. In addition, IL-4 can enhance CTL activity in MLC, but blocks IL-2 induced lymphokine activated killer cell activity in PBL. In our study, the cloning efficiencies, Ag-specific CTL activity and non-MHC-restricted cytotoxicity of CTL clones generated in IL-2 were compared to those generated in IL-4. In a first experiment, T cells were stimulated with the EBV-transformed B cell line JY and cloned 7 days later with feeder cells and either IL-2 or IL-4. In a second experiment, stimulation of the T cells was carried out in the presence of IL-2 plus anti-IL-4 antibodies or IL-4 plus anti-IL-2 antibodies in order to block the effects of IL-4 and IL-2, respectively, produced by the feeder cells. Although the cloning efficiencies in the second experiment were lower than those obtained in the first experiment, the cloning efficiencies obtained with IL-2 or IL-4 were similar in both experiments. The overall proportion of TCR alpha beta+ T cell clones cytotoxic for the stimulator cell JY established in IL-2 or IL-4 were comparable. A striking difference between the clones obtained in IL-2 or IL-4 was that a large proportion of the clones obtained in IL-4 expressed CD4 and CD8 simultaneously, whereas none of the clones isolated in IL-2 were double positive. Also gamma delta+ T cell clones could be established with IL-4 as a growth factor. TCR gamma delta+ T cell clones isolated in either IL-2 or IL-4 were CD4-CD8- or CD4-CD8+, but the proportion of CD4-CD8+ clones isolated in IL-4 was higher. Interestingly, one TCR gamma delta+ clone isolated in IL-2 was CD4+CD8-. Most of the TCR alpha beta+ and TCR gamma delta+ CTL-clones isolated in IL-2 lysed the NK cell sensitive target cell K562. In contrast, only a small proportion of the TCR alpha beta+ or TCR gamma delta+ CTL clones isolated in IL-4, lysed K562. One TCR gamma delta+ T cell clone (CD-124) isolated in IL-4 and subsequently incubated in IL-2 acquired lytic activity against K562.(ABSTRACT TRUNCATED AT 400 WORDS)
白细胞介素 -4已被证明可作为人类T细胞的生长因子。此外，白细胞介素 -4可增强混合淋巴细胞培养中的细胞毒性T淋巴细胞（CTL）活性，但会阻断外周血淋巴细胞（PBL）中白细胞介素 -2诱导的淋巴因子激活的杀伤细胞活性。在我们的研究中，比较了在白细胞介素 -2和白细胞介素 -4中产生的CTL克隆的克隆效率、抗原特异性CTL活性和非主要组织相容性复合体（MHC）限制的细胞毒性。在第一个实验中，用爱泼斯坦 - 巴尔病毒（EBV）转化的B细胞系JY刺激T细胞，并在7天后用饲养细胞和白细胞介素 -2或白细胞介素 -4进行克隆。在第二个实验中，在白细胞介素 -2加抗白细胞介素 -4抗体或白细胞介素 -4加抗白细胞介素 -2抗体的存在下刺激T细胞，以分别阻断饲养细胞产生的白细胞介素 -4和白细胞介素 -2的作用。尽管第二个实验中的克隆效率低于第一个实验，但在两个实验中，用白细胞介素 -2或白细胞介素 -4获得的克隆效率相似。在白细胞介素 -2或白细胞介素 -4中建立的对刺激细胞JY具有细胞毒性的T细胞受体αβ + T细胞克隆的总体比例相当。在白细胞介素 -2或白细胞介素 -4中获得的克隆之间的一个显著差异是，在白细胞介素 -4中获得的很大一部分克隆同时表达CD4和CD8，而在白细胞介素 -2中分离的克隆没有一个是双阳性的。以白细胞介素 -4作为生长因子也可以建立γδ + T细胞克隆。在白细胞介素 -2或白细胞介素 -4中分离的T细胞受体γδ + T细胞克隆是CD4 - CD8 - 或CD4 - CD8 +，但在白细胞介素 -4中分离的CD4 - CD8 +克隆的比例更高。有趣的是，在白细胞介素 -2中分离的一个T细胞受体γδ +克隆是CD4 + CD8 - 。在白细胞介素 -2中分离的大多数T细胞受体αβ +和T细胞受体γδ + CTL克隆裂解自然杀伤（NK）细胞敏感靶细胞K562。相比之下，在白细胞介素 -4中分离的T细胞受体αβ +或T细胞受体γδ + CTL克隆中只有一小部分裂解K562。在白细胞介素 -4中分离并随后在白细胞介素 -2中培养的一个T细胞受体γδ + T细胞克隆（CD - 124）获得了针对K562的裂解活性。（摘要截断于400字）
