Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy.
Methods Mol Biol. 2021;2285:165-172. doi: 10.1007/978-1-0716-1311-5_14.
Human T cells represent a heterogeneous population, including cells with different phenotypical and function properties. Despite, in the last years, several technologies were developed to investigate phenotypical properties of T cells at single cell level, in vitro T cell clone 's culture remains the only way to perform functional study on T cells at single cell levels. Here, we describe the method to obtain human T cell clones by limiting dilution in the presence of feeder cells and to maintain them in culture for further investigations.
人类 T 细胞是一个异质性群体，包括具有不同表型和功能特性的细胞。尽管在过去的几年中，已经开发了几种技术来研究 T 细胞的单细胞水平的表型特性，但体外 T 细胞克隆培养仍然是在单细胞水平上进行 T 细胞功能研究的唯一方法。在这里，我们描述了在饲养细胞存在的情况下通过有限稀释获得人类 T 细胞克隆的方法，并描述了如何在培养物中维持它们以供进一步研究。
