Stasiak K, Janicki B
Pol J Vet Sci. 2014;17(1):177-9. doi: 10.2478/pjvs-2014-0025.
The objective of this study was to determine the effect of age and reproductive season on selected properties of semen from the arctic fox, Aloper lagopus L. The experiment used 40 ejaculates collected manually from 6 animals (3 foxes aged one year and 3 foxes older than three years). Statistically less semen (0.39 cm3) was collected from the young compared to the older animals, and the ejaculates obtained were characterized by higher concentration of spermatozoa (195.04 x 106/cm3). In turn, sperm acrosomal extracts from the older animals contained statistically more acrosin (6,4 mU/106 spermatozoa). In the sperm acrosomal extracts prepared during the first semen sampling, the mean acrosin activity did not exceed 2.3 mU/million spermatozoa. At subsequent semen sampling dates, the activity of the analysed enzyme increased to reach 7.72 mU/million spermatozoa. In the extracts obtained from the semen collected at the end of the breeding season of arctic foxes, the acrosin activity again reached a value obtained at the beginning of the season.
本研究的目的是确定年龄和繁殖季节对北极狐（Alopex lagopus L.）精液某些特性的影响。实验采用人工采集的40份射精样本，样本来自6只动物（3只1岁的狐狸和3只3岁以上的狐狸）。与年长动物相比，年轻动物采集到的精液量在统计学上较少（0.39立方厘米），且获得的射精样本中精子浓度较高（195.04×10⁶/立方厘米）。相反，年长动物的精子顶体提取物中，在统计学上含有更多的顶体蛋白酶（6.4 mU/10⁶精子）。在首次精液采样时制备的精子顶体提取物中，顶体蛋白酶的平均活性不超过2.3 mU/百万精子。在随后的精液采样日期，所分析酶的活性增加到7.72 mU/百万精子。在北极狐繁殖季节结束时采集的精液所获得的提取物中，顶体蛋白酶活性再次达到季节开始时的数值。
