Stasiak Karolina, Cygan-Szczegielniak Dorota, Bogucka Joanna
Faculty of Animal Breeding and Biology, Bydgoszcz University of Science and Technology, Mazowiecka, Bydgoszcz, Poland.
Anim Reprod. 2021 Oct 22;18(3):e20210015. doi: 10.1590/1984-3143-AR2021-0015. eCollection 2021.
Morphology and sperm morphometry, this is an important determinant of male reproductive capacity. Morphometric data may provide relevant information in studies focused on evolutionary biology, sperm quality assessment, including prediction of the potential fertility, semen cryopreservation, or the effect of reprotoxicants. The paper presents the morphometric analysis of spermatozoa from two colour morphs of Arctic fox (Vulpes lagopus), and attempts to determine the relationship between selected quality indicators and dimensions and shape of spermatozoa. The research material consisted of ejaculates collected once by manual stimulation from 20 one-year-old Arctic foxes (10 individuals of the blue morph and 10 of the white morph). Ejaculates were analysed for standard parameters (volume, sperm concentration, total number of spermatozoa in the ejaculate) and used for the preparation of microscopic specimens. It was found that, the dimensions of spermatozoa from Arctic foxes depend on the male colour morphs. Spermatozoa from white Arctic foxes were significantly longer (by 1.82 µm) and had larger heads (0.32 µm longer and 0.15 µm wider) compared to spermatozoa from blue Arctic foxes (P<0.05). The interactions between particular sperm dimensions indicated the occurrence of gametes differing in shape. The all correlation coefficients between the morphometric traits of spermatozoa were statistically significant. Our research proved that in the blue Arctic foxes, sperm dimensions (tail length and total sperm length) can be related to the percentage of spermatozoa with primary changes (respectively: r = -0.68 and r = -0.75; at P <0.05). However, in the case of white Arctic foxes, these characteristics depend on the ejaculate volume (respectively: r = 0.65 and r = 0.68; at P <0.05).
形态学与精子形态测定，这是男性生殖能力的一个重要决定因素。形态测定数据可能会在专注于进化生物学、精子质量评估（包括潜在生育能力预测、精液冷冻保存或生殖毒性物质的影响）的研究中提供相关信息。本文呈现了对北极狐（Vulpes lagopus）两种毛色形态的精子进行的形态测定分析，并试图确定选定的质量指标与精子尺寸和形状之间的关系。研究材料包括通过手动刺激从20只一岁北极狐（10只为蓝色形态个体，10只为白色形态个体）收集的一次射精样本。对射精样本进行了标准参数分析（体积、精子浓度、射精样本中的精子总数），并用于制备显微镜标本。研究发现，北极狐精子的尺寸取决于雄性的毛色形态。与蓝色北极狐的精子相比，白色北极狐的精子明显更长（长1.82微米），头部更大（长0.32微米，宽0.15微米）（P<0.05）。特定精子尺寸之间的相互作用表明存在形状不同的配子。精子形态测定特征之间的所有相关系数均具有统计学意义。我们的研究证明，在蓝色北极狐中，精子尺寸（尾巴长度和精子总长度）可能与发生初级变化的精子百分比有关（分别为：r = -0.68和r = -0.75；P <0.05）。然而，在白色北极狐的情况下，这些特征取决于射精体积（分别为：r = 0.65和r = 0.68；P <0.05）。
