Zander R, Lang W, Wolf H U
Physiologisches Institut, Universität Mainz, FRG.
J Clin Chem Clin Biochem. 1989 Apr;27(4):185-9. doi: 10.1515/cclm.1989.27.4.185.
In order to compare the accuracy of haemoglobin (Hb) determination methods, the commonly used cyanhaemiglobin (HiCN) method and the recently developed alkaline haematin D-575 (AHD) method (R. Zander, W. Lang & H. U. Wolf (1984) Clin. Chim. Acta 136, 83-93; H. U. Wolf, W. Lang & R. Zander (1984) Clin. Chim. Acta 136, 95-104) were tested with respect to method-related errors such as plasma, cell, and Hb errors. Both methods yield a series of more or less significant errors which generally lead to an overestimation of the Hb concentration in the order of 1%. However, in all three cases of plasma errors, i.e. normal plasma error, plasma error in lipaemic blood, and plasma error in bilirubinaemic blood, the AHD method shows significantly lower values of errors than the HiCN method. In the case of cell errors such as ghost and leukocyte errors, the overestimation of the Hb concentration by the HiCN method is 60% higher than that by the AHD method. In the case of Hb errors such as fetal Hb and carboxy Hb errors, there is a significant overestimation of the Hb concentration by the HiCN method, which amounts 3 min after mixing of blood and HiCN solution to 0.7% in the case of fetal Hb and to 13.2% in the case of carboxy Hb. The latter value yields an overestimation of 1.3%, when 10% carboxy Hb in a blood sample is present. In contrast, there is no detectable overestimation after 3 min in the case of the AHD method. Thus, the AHD method provides a higher accuracy in Hb determination than the commonly used HiCN method.
为比较血红蛋白（Hb）测定方法的准确性，对常用的氰化高铁血红蛋白（HiCN）法和最近开发的碱性高铁血红素D - 575（AHD）法（R. 赞德、W. 朗和H. U. 沃尔夫（1984年）《临床化学学报》136卷，83 - 93页；H. U. 沃尔夫、W. 朗和R. 赞德（1984年）《临床化学学报》136卷，95 - 104页）就血浆、细胞和Hb误差等与方法相关的误差进行了测试。两种方法都会产生一系列或多或少显著的误差，这些误差通常会导致Hb浓度高估约1%。然而，在血浆误差的所有三种情况下，即正常血浆误差、脂血血液中的血浆误差和黄疸血中的血浆误差，AHD法显示出的误差值明显低于HiCN法。在细胞误差如影细胞和白细胞误差的情况下，HiCN法对Hb浓度的高估比AHD法高60%。在Hb误差如胎儿Hb和碳氧Hb误差的情况下，HiCN法会导致Hb浓度显著高估，血液与HiCN溶液混合3分钟后，胎儿Hb的高估幅度为0.7%，碳氧Hb的高估幅度为13.2%。当血样中存在10%的碳氧Hb时，后者的值会产生1.3%的高估。相比之下，AHD法在3分钟后没有可检测到的高估。因此，AHD法在Hb测定中比常用的HiCN法具有更高的准确性。
