van Assendelft O W, Zijlstra W G
Division of Host Factors, Centers for Disease Control, Atlanta, GA.
J Clin Chem Clin Biochem. 1989 Apr;27(4):191-5. doi: 10.1515/cclm.1989.27.4.191.
The "alkaline haematin D-575" method for determining haemoglobin, described by Zander et al. [1984) Clin. Chim. Acta 136, 83-93) was tested. Claims that different non-ionic detergents in the reagent result in identical values for the haemoglobin concentration being measured could not be verified. It could also not be verified that a stable end-product with unique spectral characteristics is always reached within approximately 2 min and that conversion of fetal haemoglobin is faster than that with the haemiglobin cyanide method. Because of the many questions regarding the nature and characteristics of the alkaline haematin/detergent complex or complexes, it is not yet possible to recommend this method for routine haemoglobinometry.
