Hall Sedlak Ruth, Jerome Keith R
Department of Laboratory Medicine, University of Washington, Seattle, Washington 98195, USA.
Expert Rev Mol Diagn. 2014 May;14(4):501-7. doi: 10.1586/14737159.2014.910456. Epub 2014 Apr 11.
Digital PCR (dPCR), a new nucleic acid amplification technology, offers several potential advantages over real-time or quantitative PCR (qPCR), the current workhorse of clinical molecular virology diagnostics. Several studies have demonstrated dPCR assays for human cytomegalovirus or HIV, which give more precise and reproducible results than qPCR assays without sacrificing sensitivity. Here we review the literature comparing dPCR and qPCR performance in viral molecular diagnostic assays and offer perspective on the future of dPCR in clinical virology diagnostics.
数字PCR（dPCR）是一种新的核酸扩增技术，与实时或定量PCR（qPCR）相比具有若干潜在优势，而qPCR是临床分子病毒学诊断中目前的主要技术。多项研究已证实针对人巨细胞病毒或HIV的dPCR检测，其结果比qPCR检测更精确且可重复，同时不牺牲灵敏度。在此，我们综述了比较dPCR和qPCR在病毒分子诊断检测中性能的文献，并对dPCR在临床病毒学诊断中的未来发展前景提出看法。
