Department of Laboratory Medicine, Peking University Third Hospital, Beijing, China.
Core Unit of National Clinical Research Center for Laboratory Medicine, Peking University Third Hospital, Beijing, China.
J Clin Lab Anal. 2024 Aug;38(15-16):e25090. doi: 10.1002/jcla.25090. Epub 2024 Aug 19.
Lower respiratory tract infection (LRTI) has long been an important threat to people's life and health, so the rapid diagnosis of LRTI is of great significance in clinical treatment. In recent years, the development of the sequencing technology provides a new direction for the rapid diagnosis of LRTI. In this review, the advantages and disadvantages of second-generation sequencing techniques represented by metagenomics next-generation sequencing (mNGS) and droplet digital polymerase chain reaction (ddPCR) in LRTI were reviewed. Furthermore, it offers insights into the future trajectory of this technology, highlighting its potential to revolutionise the field of respiratory infection diagnostics.
This review summarises developments in mechanistic research of second-generation sequencing technology their relationship with clinical practice, providing insights for future research.
Authors conducted a search on PubMed and Web of Science using the professional terms 'Lower respiratory tract infection' and 'droplet digital polymerase chain reaction' and 'metagenomics next generation sequencing'. The obtained literature was then roughly categorised based on their research content. Similar studies were grouped into the same sections, and further searches were conducted based on the keywords of each section.
Different studies discussed the application of second-generation sequencing technology in LRTI from different angles, including the detection of pathogens of LRTI by mNGS and ddPCR, the prediction ability of drug-resistant bacteria, and comparison with traditional methods. We try to analyse the advantages and disadvantages of the second-generation sequencing technology by combing the research results of mNGS and ddPCR. In addition, the development direction of the second-generation sequencing technology is prospected.
下呼吸道感染（LRTI）长期以来一直是人们生命和健康的重要威胁，因此快速诊断 LRTI 在临床治疗中具有重要意义。近年来，测序技术的发展为 LRTI 的快速诊断提供了新的方向。在本综述中，我们回顾了以宏基因组下一代测序（mNGS）和数字液滴聚合酶链反应（ddPCR）为代表的第二代测序技术在 LRTI 中的优缺点。此外，我们还探讨了该技术的未来发展轨迹，强调了其在呼吸道感染诊断领域的革命性潜力。
本综述总结了第二代测序技术的机制研究进展及其与临床实践的关系，为未来的研究提供了思路。
作者使用专业术语“下呼吸道感染”和“数字液滴聚合酶链反应”和“宏基因组下一代测序”在 PubMed 和 Web of Science 上进行了检索。然后根据文献的研究内容对文献进行了大致分类。将相似的研究归入同一部分，并根据每一部分的关键词进行进一步搜索。
不同的研究从不同的角度讨论了第二代测序技术在 LRTI 中的应用，包括 mNGS 和 ddPCR 检测 LRTI 的病原体、耐药菌的预测能力以及与传统方法的比较。我们试图通过结合 mNGS 和 ddPCR 的研究结果来分析第二代测序技术的优缺点。此外，还展望了第二代测序技术的发展方向。
