公羊精浆中蛋白质条带的存在与精子冷冻耐受性之间的关联。
Association between the presence of protein bands in ram seminal plasma and sperm tolerance to freezing.
作者信息
Goularte K L, Gastal G D A, Schiavon R S, Gonçalves A O, Schneider J R, Corcini C D, Lucia T
机构信息
ReproPel, Faculdade de Veterinária, Campus Capão do Leão, Universidade Federal de Pelotas, 96010-900 Pelotas, RS, Brazil.
ReproPel, Faculdade de Veterinária, Campus Capão do Leão, Universidade Federal de Pelotas, 96010-900 Pelotas, RS, Brazil.
出版信息
Anim Reprod Sci. 2014 May;146(3-4):165-9. doi: 10.1016/j.anireprosci.2014.03.009. Epub 2014 Mar 25.
This study evaluated associations between the presence of protein bands in ram seminal plasma and the quality of sperm frozen with distinct extenders. Ejaculates were frozen in a Tris-egg yolk based extender, including either 5% glycerol or 100mM trehalose. Seminal plasma samples were submitted to unidimensional electrophoresis. Pre-freezing and post-thawing sperm quality was similar between extenders (P>0.05). A total of 26 bands were identified in ram seminal plasma. Pre-freezing sperm motility was increased when the 15, 19 and 80kDa bands were present in seminal plasma (P<0.05). The presence of an 11kDa band in seminal plasma was associated with reduced pre-freezing membrane integrity (P<0.05). After thawing, both sperm motility and membrane integrity were reduced when a 24kDa band was present in seminal plasma (P<0.05). Post-thawing acrosome integrity was greater in the presence of a 31kDa band in seminal plasma (P<0.05). Regardless of the cryoprotectant included in the freezing extender, these six bands may be potential markers for ram sperm tolerance to freezing.
本研究评估了公羊精液中蛋白质条带的存在与用不同稀释液冷冻的精子质量之间的关联。射精样本在基于Tris-蛋黄的稀释液中冷冻，该稀释液含有5%甘油或100mM海藻糖。精液样本进行一维电泳。两种稀释液之间，冷冻前和解冻后精子质量相似（P>0.05）。在公羊精液中总共鉴定出26条带。当精液中存在15、19和80kDa条带时，冷冻前精子活力增加（P<0.05）。精液中存在11kDa条带与冷冻前膜完整性降低相关（P<0.05）。解冻后，当精液中存在24kDa条带时，精子活力和膜完整性均降低（P<0.05）。精液中存在31kDa条带时，解冻后顶体完整性更高（P<0.05）。无论冷冻稀释液中包含何种冷冻保护剂，这六条带可能是公羊精子冷冻耐受性的潜在标志物。
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