Watanabe E, Fujita S C, Murakami F, Hayashi M, Matsumura M
Department of Biophysical Engineering, Faculty of Engineering Science, Osaka University, Japan.
Neuroscience. 1989;29(3):645-57. doi: 10.1016/0306-4522(89)90137-1.
A monoclonal antibody was obtained by immunizing mice with an extract of monkey brain. The monoclonal antibody 473 stained a small subpopulation of neurons in various regions of monkey and rat central nervous system. The perimeters of neuronal somata and the proximal parts of dendrites bound the antibody. Electron microscopic analysis showed that the immunoreactivity was associated with the outer surface of the cell. The immunoreactivity in the rat cerebral cortex appeared gradually during the second four weeks after birth. The antibody stained fetal cartilages but otherwise was specific to the nervous system. Experiments on the stability of the immunoreactivity to enzymatic and chemical treatments of the sections suggest that the antigen molecule is of proteoglycan nature.
用猴脑提取物免疫小鼠获得了一种单克隆抗体。单克隆抗体473可使猴和大鼠中枢神经系统不同区域的一小部分神经元染色。神经元胞体的周边和树突的近端与该抗体结合。电子显微镜分析表明，免疫反应性与细胞外表面相关。大鼠大脑皮质中的免疫反应性在出生后的第二个四周期间逐渐出现。该抗体可使胎儿软骨染色，但除此之外对神经系统具有特异性。对切片进行酶处理和化学处理后免疫反应性稳定性的实验表明，抗原分子具有蛋白聚糖性质。
