Landis D M, Welter E, Skordeles C
Department of Neurology, School of Medicine, Case Western Reserve University, Cleveland, OH 44106.
Neuroscience. 1991;45(2):467-77. doi: 10.1016/0306-4522(91)90242-g.
Plasmalemmal fractions from cultured astrocytes have been used as the immunogen in generating a monoclonal antibody, termed 8C10, which binds to the surface of cultured astrocytes of the rat. 8C10 immunoreactivity is present on the membrane surface of cultured type 1 astrocytes, type 2 astrocytes, oligodendrocytes, meningeal cells, and 02A progenitor cells, and it persists after aldehyde fixation. The antibody also stains aldehyde-fixed central nervous system, in a pattern which suggests that the plasma membranes of fine astrocytic processes in adult neuropil express the epitope. Astrocytic perikarya and processes in white matter are also stained, but there is no immunoreactivity present in neuronal processes or perikarya. Astrocytic processes in developing cerebellar cortex are stained at postnatal ages when some of these processes are guiding the migration of neuronal perikarya.
来自培养星形胶质细胞的质膜组分已被用作免疫原，以产生一种名为8C10的单克隆抗体，该抗体可与大鼠培养星形胶质细胞的表面结合。8C10免疫反应性存在于培养的1型星形胶质细胞、2型星形胶质细胞、少突胶质细胞、脑膜细胞和02A祖细胞的膜表面，并且在醛固定后仍然存在。该抗体还能以一种表明成体神经毡中纤细星形胶质细胞突起的质膜表达该表位的模式，对醛固定的中枢神经系统进行染色。白质中的星形胶质细胞核周体和突起也被染色，但神经元突起或核周体中不存在免疫反应性。在发育中的小脑皮质中，星形胶质细胞突起在出生后的年龄段被染色，此时其中一些突起正在引导神经元核周体的迁移。
