在受城市径流影响的热带休闲海滩中出现具有毒力标记的肠球菌属物种。
Occurrence of Enterococcus species with virulence markers in an urban flow-influenced tropical recreational beach.
机构信息
School of Biosciences and Biotechnology, Faculty of Science & Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Malaysia.
School of Biosciences and Biotechnology, Faculty of Science & Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Malaysia; Institute of Ecology and Environmental Studies, Obafemi Awlowo University, Ile-Ife, Nigeria.
出版信息
Mar Pollut Bull. 2014 May 15;82(1-2):26-38. doi: 10.1016/j.marpolbul.2014.03.028. Epub 2014 Apr 13.
Median enterococci counts of beach water samples gradually increased at statistically significant levels (χ2: 26.53, df: 4; p<0.0001) with increasing proximity to river influx. The difference in proportion of antibiotic resistant enterococci in beach water and river water samples was statistically significant (p<0.05) for the tested antibiotics with river isolates generally presenting higher resistance frequencies. Virulence genes cyl, esp, gelE and asa were detected at varying frequencies (7.32%, 21.95%, 100% and 63.41% respectively) among river isolates. On the other hand, the prevalence of these genes was lower (0%, 20%, 67.27% and 41.82% respectively) among beach water isolates. Multi-Locus-Sequence-Typing analysis of Enterococcus faecalis presented four sequence types (ST) one of which shared six out of seven tested loci with ST6, a member of the clonal complex of multi-drug resistant strains associated with hospital outbreaks.
海滩水样中肠球菌的中位数计数逐渐升高，且在统计学上呈显著水平（χ2：26.53，df：4；p<0.0001），与河流流入的距离增加成正比。海滩水样和河流水样中抗生素耐药肠球菌的比例差异在测试的抗生素中具有统计学意义（p<0.05），河流分离株通常表现出更高的耐药频率。毒力基因 cyl、esp、gelE 和 asa 在河流分离株中的检出率不同（分别为 7.32%、21.95%、100%和 63.41%）。另一方面，这些基因在海滩水样分离株中的流行率较低（分别为 0%、20%、67.27%和 41.82%）。粪肠球菌的多位点序列分型分析显示了 4 种序列类型（ST），其中一种与 ST6 有 6 个测试位点相同，ST6 是与医院暴发相关的多药耐药菌株克隆复合体的成员。
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