Adeniji Oluwaseun O, Sibanda Timothy, Okoh Anthony I
SAMRC Microbial Water Quality Monitoring Centre, University of Fort Hare, Alice, 5700, South Africa.
Applied and Environmental Microbiology Research Group (AEMREG), Department of Biochemistry and Microbiology, University of Fort Hare, Private Bag X1314, Alice, 5700, Eastern Cape, South Africa.
Heliyon. 2019 Jun 21;5(6):e01893. doi: 10.1016/j.heliyon.2019.e01893. eCollection 2019 Jun.
Coastal water resources are habitually exposed to indiscriminate anthropogenic pollution. However, due to their negative consequences to the public health, recreational waters require continuous monitoring for disease-causing organisms as a way of preventing ailments associated with swimming. As a result, the present study assessed the physicochemical parameters and microbial loads of water samples collected from six different sampling points on Kidd's Beach using standard analytical procedures. Generated data were analysed with One-way ANOVA and spearman correlation (at 95%). The physicochemical qualities varied as follows: pH (7.21-8.23), temperature (18.46-27.63 °C), turbidity (0-25.67 NTU), electrical conductivity (22723-62067 μS/cm), total dissolved solids (7662-31037 mg/L), and salinity (8.95-41.84 PSU). All these measured parameters were significantly different ( < 0.05) with respect to the sampling sites. Presumptive counts ranged from 64 - 168 CFU/100 mL of water samples. Out of 409 presumptive isolates obtained from the culture-based method, 67 were confirmed to be by PCR-techniques. From the 67 confirmed isolates, 19) and 40() while 8(other species that were non-targeted). Findings from this study shown that Kidd's Beach water samples contain some pathogenic bacteria that pose high risk to the public health and make it to be unfit for recreational use when compared to DWAF and US EPA guidelines. Therefore, effort should be made to strictly control all activities contributing to the level of pollution in the marine environment.
沿海水资源经常受到任意的人为污染。然而，由于其对公众健康的负面影响，休闲水域需要持续监测致病生物，以此作为预防与游泳相关疾病的一种方式。因此，本研究采用标准分析程序，评估了从基德海滩六个不同采样点采集的水样的理化参数和微生物负荷。生成的数据用单因素方差分析和斯皮尔曼相关性分析（95%置信水平）。理化性质变化如下：pH值（7.21 - 8.23）、温度（18.46 - 27.63℃）、浊度（0 - 25.67 NTU）、电导率（22723 - 62067 μS/cm）、总溶解固体（7662 - 31037 mg/L）和盐度（8.95 - 41.84 PSU）。所有这些测量参数在采样点方面均存在显著差异（< 0.05）。推定计数范围为每100 mL水样64 - 168 CFU。从基于培养的方法获得的409个推定分离株中，有67个通过PCR技术得到确认。在这67个确认的分离株中，19个（）和40个（），而8个是其他未靶向的物种。本研究结果表明，与DWAF和美国环境保护局的指导方针相比，基德海滩的水样含有一些对公众健康构成高风险的致病细菌，使其不适用于休闲用途。因此，应努力严格控制所有导致海洋环境污染水平的活动。
