Farah Shady, Tsach Tsadok, Bentolila Alfonso, Domb Abraham J
Institute of Drug Research, School of Pharmacy-Faculty of Medicine, Center for Nanoscience and Nanotechnology and The Alex Grass Center for Drug Design and Synthesis, The Hebrew University of Jerusalem, 91120, Israel.
Division of Identification and Forensic Sciences (DIFS), Israel Police, Jerusalem, Israel.
Talanta. 2014 Jun;123:54-62. doi: 10.1016/j.talanta.2014.01.041. Epub 2014 Feb 5.
Poly(ethylene terephthalate) (PET) fiber analysis and comparison by spectral and polymer molecular weight determination was investigated. Plain fibers of PET, a common textile fiber and plastic material was chosen for this study. The fibers were analyzed for morphological (SEM and AFM), spectral (IR and NMR), thermal (DSC) and molecular weight (MS and GPC) differences. Molecular analysis of PET fibers by Gel Permeation Chromatography (GPC) allowed the comparison of fibers that could not be otherwise distinguished with high confidence. Plain PET fibers were dissolved in hexafluoroisopropanol (HFIP) and analyzed by GPC using hexafluoroisopropanol:chloroform 2:98 v/v as eluent. 14 PET fiber samples, collected from various commercial producers, were analyzed for polymer molecular weight by GPC. Distinct differences in the molecular weight of the different fiber samples were found which may have potential use in forensic fiber comparison. PET fibers with average molecular weights between about 20,000 and 70,000 g mol(-1) were determined using fiber concentrations in HFIP as low as 1 μg mL(-1). This GPC analytical method can be applied for exclusively distinguish between PET fibers using 1 μg of fiber. This method can be extended to forensic comparison of other synthetic fibers such as polyamides and acrylics.
研究了通过光谱分析和聚合物分子量测定对聚对苯二甲酸乙二酯（PET）纤维进行分析和比较。本研究选择了普通纺织纤维和塑料材料PET的普通纤维。对纤维进行了形态学（扫描电子显微镜和原子力显微镜）、光谱学（红外光谱和核磁共振）、热学（差示扫描量热法）和分子量（质谱和凝胶渗透色谱法）差异分析。通过凝胶渗透色谱法（GPC）对PET纤维进行分子分析，可以对其他方法难以高置信度区分的纤维进行比较。将普通PET纤维溶解在六氟异丙醇（HFIP）中，并使用六氟异丙醇：氯仿2:98 v/v作为洗脱剂通过GPC进行分析。对从不同商业生产商收集的14个PET纤维样品进行了GPC聚合物分子量分析。发现不同纤维样品的分子量存在明显差异，这可能在法医纤维比较中具有潜在用途。使用低至1μg mL(-1)的HFIP纤维浓度测定了平均分子量在约20,000至70,000 g mol(-1)之间的PET纤维。这种GPC分析方法可用于仅使用1μg纤维区分PET纤维。该方法可扩展到对其他合成纤维如聚酰胺和腈纶的法医比较。
