Departments of Molecular Microbiology and Pathology & Immunology, Washington University School of Medicine, St. Louis, MO, USA.
Department of Pediatrics, Washington University School of Medicine, St. Louis, MO, USA.
Virology. 2014 Apr;454-455:25-33. doi: 10.1016/j.virol.2014.01.018. Epub 2014 Feb 20.
We describe the identification of a novel picornavirus recovered from the fecal specimen of a child in The Gambia, provisionally named rosavirus 2. Comparison of the rosavirus 2 complete genome demonstrated 71.9% nucleotide identity to its closest relative rosavirus M-7, an unclassified picornavirus identified from rodent fecal material. A unique RNA structure was predicted in the 3' UTR of rosavirus 2 that was conserved with rosavirus M-7 and cadiciviruses. We detected rosavirus 2 in four pediatric fecal specimens (0.55% prevalence) in a Gambian diarrheal case-control cohort, but we did not detect it in a panel of 634 pediatric diarrheal stool specimens from the USA. There was no statistical evidence that rosavirus 2 was associated with diarrheal cases. This study broadens our understanding of unknown viruses present in children in developing country settings.
我们描述了一种从冈比亚儿童粪便标本中分离出的新型微小核糖核酸病毒，暂时命名为玫瑰病毒 2。对玫瑰病毒 2 全基因组的比较表明，其核苷酸同源性为 71.9%，与最接近的亲缘病毒玫瑰病毒 M-7 同源，玫瑰病毒 M-7 是一种从啮齿动物粪便中分离出的未分类微小核糖核酸病毒。在玫瑰病毒 2 的 3'UTR 中预测到了一个独特的 RNA 结构，该结构与玫瑰病毒 M-7 和 CAD 病毒保守。我们在冈比亚腹泻病例对照队列中的四个儿科粪便标本中检测到了玫瑰病毒 2（0.55%的流行率），但在美国的 634 个儿科腹泻粪便标本中没有检测到。没有统计学证据表明玫瑰病毒 2 与腹泻病例有关。本研究拓宽了我们对发展中国家儿童中存在的未知病毒的认识。
