Siriraj Influenza Cooperative Research Center, Department of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok-noi, Bangkok 10700, Thailand; Center for Emerging and Neglected Infectious Disease, Mahidol University, Nakhon Pathom 73170, Thailand.
Siriraj Influenza Cooperative Research Center, Department of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok-noi, Bangkok 10700, Thailand.
Virology. 2014 Apr;454-455:254-62. doi: 10.1016/j.virol.2014.02.024. Epub 2014 Mar 21.
Six recombinant vaccinia viruses containing HA, NA, NP, M or NS gene insert derived from a highly pathogenic avian influenza H5N1 virus, and the recombinant vaccinia virus harboring plasmid backbone as the virus control were constructed. The recombinant proteins were characterized for their expression and subcellular locations in TK(-) cells. Antibodies to the five recombinant proteins were detected in all 13 sequential serum samples collected from four H5N1 survivors during four years of follow-up; and those directed to rVac-H5 HA and rVac-NA proteins were found in higher titers than those directed to the internal proteins as revealed by indirect immunofluorescence assay. Although all 28 non-H5N1 subjects had no neutralizing antibodies against H5N1 virus, they did have cross-reactive antibodies to those five recombinant proteins. A significant increase in cross-reactive antibody titer to rVac-H5 HA and rVac-NA was found in paired blood samples from patients infected with the 2009 pandemic virus.
构建了包含源自高致病性禽流感 H5N1 病毒的 HA、NA、NP、M 或 NS 基因插入物的 6 种重组痘苗病毒，以及携带质粒骨架作为病毒对照的重组痘苗病毒。在 TK(-)细胞中对重组蛋白的表达和亚细胞定位进行了特征分析。在对 4 名 H5N1 幸存者在 4 年随访期间收集的 13 份连续血清样本中检测到了针对这 5 种重组蛋白的抗体；间接免疫荧光检测显示，针对 rVac-H5 HA 和 rVac-NA 蛋白的抗体滴度高于针对内部蛋白的抗体滴度。尽管所有 28 名非 H5N1 受试者均未针对 H5N1 病毒产生中和抗体，但他们对这 5 种重组蛋白均具有交叉反应性抗体。从感染 2009 年大流行病毒的患者的配对血样中发现，针对 rVac-H5 HA 和 rVac-NA 的交叉反应性抗体滴度显著增加。
