Graduate Institute of Basic Medical Science, China Medical University, Taichung, Taiwan, ROC; Puli Branch, Taichung Veterans General Hospital, Nantou, Taiwan, ROC.
Graduate Institute of Basic Medical Science, China Medical University, Taichung, Taiwan, ROC.
Cancer Lett. 2014 Jul 10;349(1):51-60. doi: 10.1016/j.canlet.2014.03.023. Epub 2014 Apr 12.
Chemotherapy is usually applied to treat colon cancer but leads to chemoresistance, and increased metastasis and invasion. The main focus of this study is to observe effects of resistance to irinotecan (CPT-11) on metastasis, invasion and autophagy in CPT-11 resistant (CPT-11-R) LoVo colon cancer cells. CPT-11, a topoisomerase I inhibitor and a first-line chemotherapeutic drug, is used to treat colon cancer. CPT-11-R cells were constructed in a step-wise fashion with increasing CPT-11 doses. The CPT-11-R strain had a significantly lower expression of Wnt/β-catenin pathway, but induced an EGFR/IKKα/β/NF-κB pathway with elevated cell cycle, metastasis and basal autophagy.
化疗通常用于治疗结肠癌，但会导致化疗耐药性，并增加转移和侵袭。本研究的主要重点是观察伊立替康（CPT-11）耐药性对 CPT-11 耐药（CPT-11-R）LoVo 结肠癌细胞转移、侵袭和自噬的影响。CPT-11 是拓扑异构酶 I 抑制剂和一线化疗药物，用于治疗结肠癌。通过逐步增加 CPT-11 剂量构建 CPT-11-R 细胞。CPT-11-R 株中 Wnt/β-catenin 通路表达明显降低，但诱导 EGFR/IKKα/β/NF-κB 通路，细胞周期、转移和基础自噬增加。
