Departamento de Fisiología, Facultad de Medicina, Universidad Autónoma de Nuevo León, Monterrey, Nuevo León, Mexico.
Departamento de Fisiología, Facultad de Medicina, Universidad Autónoma de Nuevo León, Monterrey, Nuevo León, Mexico.
Arch Med Res. 2014 Apr;45(3):210-6. doi: 10.1016/j.arcmed.2014.02.001. Epub 2014 Apr 13.
Temporal occlusion of the hepatoduodenal ligament (HDL) is often used during liver surgeries in order to reduce blood loss, resulting in ischemia/reperfusion injury (I/R). The aim of the study was to investigate the effects of atorvastatin (ATOR) on hepatic I/R injury and on serum levels of tumor necrosis factor-alpha (TNF-α), endothelin-1 (ET-1), antithrombin III (ATIII) and intracellular adhesion molecule-1 (ICAM-1).
Liver ischemia was induced in Wistar rats by clamping the HDL for 60 min, followed by either 60 or 180 min reperfusion. Rats received either vehicle or 10 mg/kg ATOR before hepatic I/R. Control group received sham surgery. Livers were examined for histological damage and serum AST, ALT, TNF-α, ET-1, ATIII and ICAM-1 concentrations were measured.
After I/R, AST and ALT were significantly elevated, ATIII levels were significantly depleted, both TNF-α and ICAM-1 levels increased and ET-1 was significantly elevated (at 180 min). ATOR pretreatment attenuated these alterations and diminished histological injury scores.
Our results show that ATOR protects the liver from I/R injury.
在肝脏手术中，常采用阻断肝十二指肠韧带（HDL）的方法来减少失血，由此导致肝脏缺血再灌注损伤（I/R）。本研究旨在探讨阿托伐他汀（ATOR）对肝脏 I/R 损伤及血清肿瘤坏死因子-α（TNF-α）、内皮素-1（ET-1）、抗凝血酶 III（ATIII）和细胞间黏附分子-1（ICAM-1）水平的影响。
采用夹闭 HDL 60 min 再灌注 60 或 180 min 的方法诱导 Wistar 大鼠肝脏缺血，然后分别给予载体或 10 mg/kg ATOR。对照组行假手术。检测肝脏组织学损伤，并测定血清 AST、ALT、TNF-α、ET-1、ATIII 和 ICAM-1 浓度。
I/R 后，AST 和 ALT 显著升高，ATIII 水平显著下降，TNF-α和 ICAM-1 水平升高，ET-1 水平升高（180 min 时）。ATOR 预处理可减轻这些变化，降低组织学损伤评分。
我们的结果表明，ATOR 可减轻肝脏 I/R 损伤。
