Department of Medical Biosciences, Pathology, Umeå University, Umeå, Sweden.
Department of Pathology and Cytology, Karolinska University Hospital, Stockholm, Sweden.
Eur J Cancer. 2014 Jul;50(10):1829-1835. doi: 10.1016/j.ejca.2014.03.278. Epub 2014 Apr 10.
To elucidate if the density of inflammatory cells expressing S100A9 in malignant and surrounding non-malignant prostate tissues is a prognostic marker for outcome in prostate cancer patients.
Tissue was obtained from 358 men diagnosed with prostate cancer at transurethral resection of the prostate due to obstructive voiding problems, of which 260 were then followed with watchful waiting. Tissue microarrays of both malignant and non-malignant tissues were stained with an antibody against S100A9. The number of stained inflammatory cells was scored and related to clinical outcome and histopathological parameters of known prognostic value.
A high number of inflammatory cells expressing S100A9 in both malignant and surrounding non-malignant tissues were associated with significantly shorter cancer-specific survival. This association remained significant when Gleason score and local tumour stage were analysed together with S100A9 in a Cox regression model. Low number of S100A9 positive cells in non-malignant stroma was correlated to significantly longer cancer-specific survival also in patients with Gleason score 8-10 tumours. S100A9 positive cells in tumour stroma were correlated with Gleason score, hyaluronan, platelet-derived growth factor receptor beta (PDGFR-β), and androgen receptor (inverse correlation) in tumour stroma. S100A9 positive cells in non-malignant stroma correlated with androgen receptor in this tissue compartment (inverse correlation).
A high number of S100A9 positive inflammatory cells in tumour stroma and in non-malignant stroma were associated with shorter cancer-specific survival in prostate cancer patients.
阐明在恶性和周围非恶性前列腺组织中表达 S100A9 的炎症细胞密度是否是前列腺癌患者预后的标志物。
从因排尿困难而接受经尿道前列腺切除术的 358 名前列腺癌患者中获得组织，其中 260 名随后进行了观察等待。用针对 S100A9 的抗体对恶性和非恶性组织的组织微阵列进行染色。对染色的炎症细胞数量进行评分，并与已知具有预后价值的临床结果和组织病理学参数相关联。
在恶性和周围非恶性组织中表达 S100A9 的大量炎症细胞与癌症特异性生存率显著缩短相关。当在 Cox 回归模型中同时分析 Gleason 评分和局部肿瘤分期以及 S100A9 时，这种相关性仍然显著。非恶性基质中 S100A9 阳性细胞数量较少与 Gleason 评分 8-10 肿瘤患者的癌症特异性生存率显著延长相关。肿瘤基质中的 S100A9 阳性细胞与 Gleason 评分、透明质酸、血小板衍生生长因子受体-β (PDGFR-β) 和肿瘤基质中的雄激素受体（负相关）相关。非恶性基质中的 S100A9 阳性细胞与该组织区室中的雄激素受体呈负相关。
肿瘤基质和非恶性基质中 S100A9 阳性炎症细胞数量较多与前列腺癌患者的癌症特异性生存率缩短相关。
