Laboratory of Biotechnology, Institute of Animal Nutrition, Northeast Agricultural University, Harbin 150030, China.
Laboratory of Biotechnology, Institute of Animal Nutrition, Northeast Agricultural University, Harbin 150030, China.
J Microbiol Methods. 2014 Jun;101:70-80. doi: 10.1016/j.mimet.2014.03.017. Epub 2014 Apr 12.
To hydrolyze blood meal (BM) effectively, two mutants were generated using ultra-violet mutagenesis. Single-factor experiments, the Plackett-Burman technique and response surface methodology were used to optimize the fermentation conditions. This study successfully generated a mutant and innovatively provided important parameters for utilizing BM by fermentation, which could be of industrial value.
为了有效地水解血粉(BM),本研究使用超紫外线诱变技术生成了两种突变体。通过单因素实验、Plackett-Burman 技术和响应面法优化了发酵条件。本研究成功生成了一种突变体,并为发酵利用 BM 提供了重要的参数,具有工业价值。