Uchida H, Nakayama I, Noguchi S
First Department of Pathology, Medical College of Oita, Japan.
Acta Pathol Jpn. 1989 Mar;39(3):169-75. doi: 10.1111/j.1440-1827.1989.tb01496.x.
Intermediate filaments in benign and malignant thyroid lesions were immunohistochemically studied using polyclonal and monoclonal anti-cytokeratin (CK), and monoclonal anti-vimentin antibodies. Antigenicity of CK and vimentin was almost completely destroyed during formalin fixation in normal thyroid and all thyroid lesions except for some cases of papillary and squamous cell carcinoma, although the latter showed negative immunostaining with anti-vimentin antibody. In sections fixed with Carnoy's fixative, most cases of papillary carcinoma showed an intense reaction product for polyclonal anti-CK, monoclonal anti-CK-7, CK-19 and anti-vimentin antibodies. The reaction product for anti-CK antibodies was located mainly in the apical cytoplasm and that for anti-vimentin antibody in the basal cytoplasm. However antigenicity was still destroyed by the fixative in many specimens of normal thyroid, benign thyroid lesions and follicular carcinoma. In frozen sections, all specimens showed preserved antigenicity for both antigens with an intense reaction product in papillary carcinoma, but this was weaker in normal thyroid, benign thyroid lesions and follicular carcinoma. Therefore, follicular cells under normal and pathological conditions contain intermediate filaments of CK and vimentin in their cytoplasm and co-expression of the antigens is significantly increased in papillary carcinoma.
使用多克隆和单克隆抗细胞角蛋白（CK）以及单克隆抗波形蛋白抗体，对良性和恶性甲状腺病变中的中间丝进行了免疫组织化学研究。在正常甲状腺以及除某些乳头状癌和鳞状细胞癌病例外的所有甲状腺病变中，福尔马林固定过程几乎完全破坏了CK和波形蛋白的抗原性，尽管后者用抗波形蛋白抗体显示阴性免疫染色。在用卡诺氏固定液固定的切片中，大多数乳头状癌病例对多克隆抗CK、单克隆抗CK-7、CK-19和抗波形蛋白抗体显示强烈反应产物。抗CK抗体的反应产物主要位于顶端细胞质中，抗波形蛋白抗体的反应产物位于基底细胞质中。然而，在许多正常甲状腺、良性甲状腺病变和滤泡癌标本中，固定液仍会破坏抗原性。在冰冻切片中，所有标本对这两种抗原均显示保留的抗原性，乳头状癌中有强烈反应产物，但在正常甲状腺、良性甲状腺病变和滤泡癌中较弱。因此，正常和病理条件下的滤泡细胞在其细胞质中含有CK和波形蛋白的中间丝，并且在乳头状癌中抗原的共表达显著增加。
