Alam S, Weigand W A, Hong J
Department of Chemical Engineering, Illinois Institute of Technology, Chicago 60616.
Appl Biochem Biotechnol. 1989 Jan-Aug;20-21:421-36. doi: 10.1007/BF02936500.
The growth of Bacillus amyloliquefaciens in the aqueous two-phase system, made up of polyethylene glycol, dextran, and water, was investigated. Generally, Bacillus partitions in the dextran phase, but the magnitude of the separation depends largely on the overall composition of polymers in the phase system. The kinetics of growth of Bacillus amyloliquefaciens was studied in the polyethylene glycol-rich continuous phase, dextran-rich dispersed phase, and in the mixed phase. From the kinetic data it appears that increasing the overall polymer composition causes the cells to adsorp at the interface. On the other hand, partition measurements indicate that increasing polymer concentrations make the cell partitioning more one-sided. This anomaly is explained by studying the interfacial adsorption of cells via dynamic surface tension measurements.
研究了解淀粉芽孢杆菌在由聚乙二醇、葡聚糖和水组成的双水相体系中的生长情况。通常，芽孢杆菌会分配到葡聚糖相中，但分离程度在很大程度上取决于相体系中聚合物的总体组成。在富含聚乙二醇的连续相、富含葡聚糖的分散相以及混合相中研究了解淀粉芽孢杆菌的生长动力学。从动力学数据来看，增加聚合物的总体组成会导致细胞吸附在界面上。另一方面，分配测量表明，增加聚合物浓度会使细胞分配更加偏向一侧。通过动态表面张力测量研究细胞的界面吸附来解释这种异常现象。
