Hellio R, Guibourdenche M, Collatz E, Riou J Y
Département de Biologie Moléculaire, Institut Pasteur, Paris.
Ann Inst Pasteur Microbiol. 1988 Sep-Oct;139(5):515-25. doi: 10.1016/0769-2609(88)90151-2.
The envelope structure of Branhamella catarrhalis was studied by electron microscopy and compared with that of other bacteria of the family Neisseriaceae, such as Moraxella lacunata subsp. liquefaciens and Neisseria gonorrhoeae. Negative staining of B. catarrhalis showed a mamilliform surface similar to that of Moraxella. On thin sections, the cell wall appeared to be made up of a wavy outer membrane tightly linked to a straight peptidoglycan layer. Spicule-like structures protruded from the cell surface. Ruthenium red staining revealed that they contained polysaccharides. While the outer polysaccharide layer of N. gonorrhoeae was unstable after repeated subcultures in vitro, this layer remained stable in B. catarrhalis and in Moraxella lacunata subsp. liquefaciens.
通过电子显微镜对卡他布兰汉菌的包膜结构进行了研究，并与奈瑟菌科的其他细菌，如液化莫拉菌亚种和淋病奈瑟菌进行了比较。卡他布兰汉菌的负染色显示出与莫拉菌相似的乳头状表面。在超薄切片上，细胞壁似乎由紧密连接到直的肽聚糖层的波浪状外膜组成。针状结构从细胞表面突出。钌红染色显示它们含有多糖。虽然淋病奈瑟菌的外多糖层在体外反复传代后不稳定，但该层在卡他布兰汉菌和液化莫拉菌亚种中保持稳定。
