Ikegbunam Moses Nkechukwu, Anagu Linda Onyeka, Iroha Ifeanyi R, Ejikeugwu Chika Ebiye, Esimone Charles Okey
Departments of Pharmaceutical microbiology and Biotechnology, Faculty of Pharmaceutical Sciences, Nnamdi Azikiwe University, Awka, Anambra State, Nigeria.
Departments of Pharmaceutical microbiology and Biotechnology, Faculty of Pharmaceutical Sciences, Nnamdi Azikiwe University, Awka, Anambra State, Nigeria; Department of Applied Microbiology, Faculty of Biological Sciences, Ebonyi State University, Abakaliki, Ebonyi State, Nigeria.
PLoS One. 2014 Apr 11;9(4):e94461. doi: 10.1371/journal.pone.0094461. eCollection 2014.
In this study, the presence of extended spectrum beta lactamase (ESBL) producing organisms in abattoirs, a non-hospital community was investigated. The presence of ESBL-producing phenotypes was confirmed by the Double Disc Synergy Test (DDST). Out of the 99 isolates screened for ESBL, 28 (28.3%) were confirmed positive. The positive isolates were characterised by using Matrix-Assisted Laser Desorption/Ionization Time of flight Mass Spectrometry. 50% of the isolates were Pseudomonas spp., the rest were different species of Acinetobacter, Stenotrophomonas and Achromobacter. Pseudomonas monteilli and Pseudomonas putida were the most occurring in the intestine. The entire positive ESBL producers were subjected to plasmid curing to ascertain the location of the resistant marker. The result of the plasmid curing indicated that the resistant genes were chromosomally borne. The findings have therefore established the presence of ESBL producing organisms in the gut of animals from abattoirs and the table were the meat are sold, and its rate of occurrence is comparable to hospital ICUs. Abattoir communities could probably be a source of human infection with ESBL expressing pathogens and possible transfer to non-ESBL producers.
在本研究中，对非医院环境的屠宰场中产生超广谱β-内酰胺酶（ESBL）的微生物的存在情况进行了调查。通过双纸片协同试验（DDST）确认了产生ESBL表型的存在。在筛选ESBL的99株分离物中，28株（28.3%）被确认为阳性。通过基质辅助激光解吸/电离飞行时间质谱对阳性分离物进行了鉴定。50%的分离物为假单胞菌属，其余为不动杆菌属、嗜麦芽窄食单胞菌属和无色杆菌属的不同种。蒙氏假单胞菌和恶臭假单胞菌在肠道中最为常见。对所有阳性ESBL产生菌进行质粒消除以确定抗性标记的位置。质粒消除结果表明抗性基因是染色体携带的。因此，这些发现证实了在屠宰场动物肠道以及肉类销售场所存在产生ESBL的微生物，其发生率与医院重症监护病房相当。屠宰场环境可能是人类感染表达ESBL病原体并可能将其传播给非ESBL产生菌的一个来源。
