Asahikawa Medical University, 064-0821, Sapporo, N-1W-25 Hokkaido, Japan.
Hokkaido University, 064-0821, Sapporo, Hokkaido N-1W-25, Japan.
Adv Exp Med Biol. 2014;812:361-368. doi: 10.1007/978-1-4939-0620-8_48.
Vascular surgery for distal vein arterialization (DVA) has been adopted clinically as a strategy for saving arteriosclerotic lower limbs from amputation. To gain more detailed information on DVA, the present study investigated the procedure in hind limbs of rats under isoflurane anesthesia. Since successful DVA requires destruction of venous valves, a coronary angioplasty catheter guidewire was used to destroy valves either solely in the femoral vein or in both femoral and popliteal veins. The femoral artery was then anastomosed to the femoral vein with sutures under binocular microsopic control. Changes in the distribution of skin blood flow in the hind limbs were studied with a thermal camera. Skin temperature increased in the thigh and knee after femoral venous valve destruction, but hyperthermia was observed in the distal leg and foot only when the valves in the popliteal vein were also disrupted. These results showed that increased arterial blood flow could be established by DVA surgery in both the proximal and distal regions of the hind limbs.
血管外科学中的远端静脉动脉化（DVA）已被临床采用，作为一种策略来避免动脉硬化性下肢截肢。为了更详细地了解 DVA，本研究在异氟烷麻醉的大鼠后肢中研究了该手术。由于成功的 DVA 需要破坏静脉瓣膜，因此使用冠状动脉成形术导管导丝仅破坏股静脉或股静脉和膕静脉中的瓣膜。然后在双目显微镜控制下用缝线将股动脉吻合到股静脉上。使用热像仪研究了后肢皮肤血流分布的变化。股静脉瓣膜破坏后大腿和膝盖的皮肤温度升高，但只有当膕静脉中的瓣膜也被破坏时，才会观察到小腿和足部的高热。这些结果表明，通过 DVA 手术可以在下肢的近端和远端区域建立增加的动脉血流。
