Steinitz Hadass, Sadeh Adi, Kliot Adi, Harari Ally
Department of Entomology, Volcani Centre, Israel.
Pest Manag Sci. 2015 Jan;71(1):24-31. doi: 10.1002/ps.3797. Epub 2014 May 27.
The sterile insect technique (SIT) is an alternative, environmentally friendly method for controlling insect pests. In the Lepidoptera, a low dose of gamma irradiation causes inherited sterility (SIT-IS), leading to full sterility in females but only partial sterility in males, which successfully compete with wild males for mates. This study examined the effect of a low radiation dose (150 Gy) on the fitness parameters of male and female Lobesia botrana, a polyphagous and major pest of vineyards found in the Middle East, Europe and the Americas.
Irradiation of the pupae did not affect their emergence rate, flight ability out of a cylinder, male response to sex pheromone in a field cage or male or female mating success. A major effect of irradiation was observed in the significantly reduced number of irradiated females' offspring reaching pupation, and as a consequence a limited number of F2 offspring. The effect of irradiation on male partial sterility (also called inherited sterility) was reflected in the male-biased sex ratio of F1 offspring of irradiated males, the reduced number of F1 offspring and the very low number of F2 descendants.
This study demonstrates the feasibility of controlling L. botrana using SIT-IS. Adding this method to the arsenal of environmentally friendly tools to control this pest may assist in further reducing the use of insecticides on edible crops.
昆虫不育技术（SIT）是一种用于控制害虫的环保替代方法。在鳞翅目中，低剂量的伽马辐射会导致遗传性不育（SIT-IS），使雌性完全不育，但雄性仅部分不育，这些雄性仍能成功与野生雄性竞争配偶。本研究考察了低辐射剂量（150戈瑞）对葡萄小卷蛾雌雄个体适合度参数的影响，葡萄小卷蛾是一种多食性害虫，在中东、欧洲和美洲的葡萄园均有发现。
蛹期辐照并未影响其羽化率、从圆筒中飞出的飞行能力、田间网笼中雄性对性信息素的反应或雌雄个体的交配成功率。辐照的一个主要影响是，受辐照雌性后代进入蛹期的数量显著减少，因此F2代后代数量有限。辐照对雄性部分不育（也称为遗传性不育）的影响体现在受辐照雄性F1代后代的雄性偏多的性别比例、F1代后代数量减少以及F2代后代数量极少。
本研究证明了使用SIT-IS控制葡萄小卷蛾的可行性。将此方法添加到控制该害虫的环保工具库中，可能有助于进一步减少食用作物上杀虫剂的使用。
