Cai Zhaohui, Yao Zhichao, Li Yushan, Xi Zhiyong, Bourtzis Kostas, Zhao Zheng, Bai Shuai, Zhang Hongyu
State Key Laboratory of Agricultural Microbiology Key Laboratory of Horticultural Plant Biology (MOE) China-Australia Joint Research Centre for Horticultural and Urban Pests Institute of Urban and Horticultural Entomology College of Plant Science and Technology Huazhong Agricultural University Wuhan China.
Department of Microbiology and Molecular Genetics Michigan State University East Lansing Michigan.
Evol Appl. 2018 Oct 9;11(10):1946-1963. doi: 10.1111/eva.12698. eCollection 2018 Dec.
The sterile insect technique (SIT) as an eco-friendly and reliable strategy has been used to control populations of insect pests of agricultural, veterinary and human health importance. Successful applications of SIT rely on the high-level ecological fitness of sterile males. A suitable and stable gut microbiome can contribute to the ecological fitness of insect by influencing their physiology, biochemistry and development processes. Here, we show that a shift in the gut bacterial composition and structure by sterilizing irradiation, characterized by a decrease in the major gut microbiota community Enterobacteriaceae, an expansion of the minor members (e.g., Bacillaceae) and a higher richness and diversity, is tightly linked to radiation-induced ecological fitness (male mating competitiveness, flight capacity, survival rate and life span) decline in (Hendel) sterile males. Function prediction of gut microbiota indicated that changes in microbiome taxonomy tend to drive microbiome functional shifts. A higher nutrient consumption of the flourishing minor gut microbiota may cause a decline in nutrients and energy metabolic activity of host and then result in the reduced ecological fitness of irradiated flies. Furthermore, we found that a gut bacterial strain (BD177) can restore ecological fitness by improving food intake and increasing haemolymph sugar and amino acid levels of irradiated flies. Our findings suggest that gut symbiont-based probiotics can be used as agents for reversing radiation-induced ecological fitness decrease.
昆虫不育技术（SIT）作为一种生态友好且可靠的策略，已被用于控制对农业、兽医和人类健康具有重要意义的害虫种群。SIT的成功应用依赖于不育雄虫的高水平生态适应性。合适且稳定的肠道微生物群可通过影响昆虫的生理、生化和发育过程，促进其生态适应性。在此，我们表明，以肠道主要微生物群落肠杆菌科减少、次要成员（如芽孢杆菌科）扩张以及更高的丰富度和多样性为特征的、由绝育辐射导致的肠道细菌组成和结构变化，与辐射诱导的亨德尔（Hendel）不育雄虫生态适应性（雄虫交配竞争力、飞行能力、存活率和寿命）下降紧密相关。肠道微生物群的功能预测表明，微生物群落分类学的变化往往会推动微生物群落功能的转变。蓬勃发展的次要肠道微生物群对营养物质的更高消耗可能会导致宿主营养物质和能量代谢活动下降，进而导致受辐射果蝇的生态适应性降低。此外，我们发现一种肠道细菌菌株（BD177）可通过改善受辐射果蝇的食物摄入量以及提高其血淋巴糖和氨基酸水平来恢复生态适应性。我们的研究结果表明，基于肠道共生菌的益生菌可作为逆转辐射诱导的生态适应性下降的制剂。
