Hadi Najah R, Yusif Fadhil G, Yousif Maitham, Jaen Karrar K
Department of Pharmacology and Therapeutics, College of Medicine, Kufa University, P.O. Box 83, Kufa, Iraq.
Department of Surgery, College of Medicine, Kufa University, Kufa, Iraq.
ISRN Pharmacol. 2014 Mar 4;2014:206951. doi: 10.1155/2014/206951. eCollection 2014.
Although reperfusion of an ischemic organ is essential to prevent irreversible tissue damage, it may amplify tissue injury. This study investigates the role of endogenous testosterone in myocardial ischemia reperfusion and apoptosis in male rats. Material and method. Twenty four male rats were randomized into 4 equal groups: Group (1), sham group, rats underwent the same anesthetic and surgical procedure as the control group except for LAD ligation; Group (2), Active control group, rats underwent LAD ligation; Group (3), castrated, rats underwent surgical castration, left 3wks for recovery, and then underwent LAD ligation; and Group (4), Goserelin acetate treated, rats received 3.6 mg of Goserelin 3 wks before surgery and then underwent LAD ligation. At the end of experiment, plasma cTn I, cardiac TNF- α , IL1- β , ICAM-1, and Apoptosis level were measured and histological examination was made. Results. Compared to sham group, the levels of myocardial TNF- α , IL-1 β , ICAM-1, apoptosis, and plasma cTn I were significantly increased (P < 0.05) in control group and all rats showed significant myocardial injury (P < 0.05). Castration and Goserelin acetates significantly counteract the increase in myocardial levels of TNF- α , IL-1 β , ICAM-1, plasma cTn I, and apoptosis (P < 0.05) and significantly reduce (P < 0.05) the severity of myocardial injury. We conclude that castration and Goserelin acetates ameliorate myocardial I/R injury and apoptosis in rats via interfering with inflammatory reactions.
尽管缺血器官的再灌注对于预防不可逆的组织损伤至关重要，但它可能会加剧组织损伤。本研究调查内源性睾酮在雄性大鼠心肌缺血再灌注及细胞凋亡中的作用。材料与方法。24只雄性大鼠被随机分为4组，每组数量相等：第1组为假手术组，大鼠接受与对照组相同的麻醉和手术操作，但不进行左前降支（LAD）结扎；第2组为阳性对照组，大鼠进行LAD结扎；第3组为去势组，大鼠接受手术去势，术后恢复3周，然后进行LAD结扎；第4组为醋酸戈舍瑞林治疗组，大鼠在手术前3周接受3.6mg醋酸戈舍瑞林，然后进行LAD结扎。实验结束时，测定血浆肌钙蛋白I（cTn I）、心脏肿瘤坏死因子-α（TNF-α）、白细胞介素1-β（IL1-β）、细胞间黏附分子-1（ICAM-1）水平及细胞凋亡水平，并进行组织学检查。结果。与假手术组相比，对照组心肌TNF-α、IL-1β、ICAM-1、细胞凋亡水平及血浆cTn I水平显著升高（P<0.05），所有大鼠均表现出明显的心肌损伤（P<0.05）。去势和醋酸戈舍瑞林显著对抗心肌TNF-α、IL-1β、ICAM-1、血浆cTn I水平及细胞凋亡的升高（P<0.05），并显著减轻（P<0.05）心肌损伤的严重程度。我们得出结论，去势和醋酸戈舍瑞林通过干扰炎症反应减轻大鼠心肌缺血/再灌注损伤及细胞凋亡。
