[酿酒酵母细胞中逆转录产物的检测]
[detection of products of reverse transcription in Saccharomyces cells].
作者信息
Ratovitskiĭ E A
出版信息
Genetika. 1989 May;25(5):819-28.
PMID:2473004
Abstract
Poly(A)-RNA-DNA hybrids complementary to retrotransposons Ty1 have been isolated from baker yeast cells. DNA and RNA in these complexes are represented by perfect hybrids. Reverse transcription enzymatic activity was identified in yeast cells. The results are consistent with the model of reverse transcriptional replication and transposition of Ty elements, similar to that of retroviruses.
摘要
已从面包酵母细胞中分离出与逆转座子Ty1互补的聚腺苷酸RNA-DNA杂交体。这些复合物中的DNA和RNA由完美的杂交体代表。在酵母细胞中鉴定出逆转录酶活性。结果与Ty元件的逆转录复制和转座模型一致，类似于逆转录病毒。
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