Morka Katarzyna, Banaszkiewicz Sylwia, Korkus Jakub, Bania Jacek, Bystroń Jarosław, Bugla-Płoskońska Gabriela, Stanek Marta, Sokalska Urszula, Szymczyk-Nużka Małgorzata, Sheppard Samuel K, Pascoe Ben
Department of Food Hygiene and Consumer Health Protection, Wrocław University of Environmental and Life Sciences, Norwida Str. 31, 50-375 Wrocław, Poland.
Department of Microbiology, Faculty of Biological Sciences, University of Wroclaw, Przybyszewskiego 63 St., 51-148 Wrocław, Poland.
Int J Mol Sci. 2025 Jun 16;26(12):5775. doi: 10.3390/ijms26125775.
Red blood cell (RBC) concentrates remain at risk of bacterial contamination during cold storage. Although infrequent, poses a significant blood safety risk. This study aimed to assess bioserotype growth in RBC concentrates, serum sensitivity, and genetic diversity including iron metabolism genes. Ten isolates from bioserotypes 1A, 1B/O:8, 4/O:3, and 2/O:9 were incubated in RBC concentrates and counted on days 3, 7, 14, 21, and 28. After incubation, the isolates were tested in human serum (NHS). Eight genomes were sequenced, analyzed using cgMLST, and screened for iron metabolism genes. The isolates formed two clusters, with 186dz (1A) and Ye8 (1B/O:8) as singletons. After 28 days in the RBC concentrates, the bacterial counts ranged from 1.98 × 10⁵ to 1.2 × 10⁹ CFU/mL, with Ye8 (1B/O:8) achieving the highest growth and one 4/O:3 isolate showing the lowest. All isolates survived 15-30 min in NHS, but the 28s isolate did not survive at 60 min. Serum sensitivity increased in two isolates, decreased in three, and remained unchanged in five. Isolates contained 27-42 iron metabolism genes with multiple allelic variants. The iron metabolism gene content or variants may influence the growth of in RBC.
红细胞（RBC）浓缩物在冷藏期间仍有细菌污染的风险。虽然这种情况不常见，但却构成了重大的血液安全风险。本研究旨在评估红细胞浓缩物中生物血清型的生长情况、血清敏感性以及包括铁代谢基因在内的遗传多样性。将来自生物血清型1A、1B/O:8、4/O:3和2/O:9的10株分离株在红细胞浓缩物中培养，并在第3、7、14、21和28天进行计数。培养后，将分离株在人血清（NHS）中进行测试。对8个基因组进行测序，使用cgMLST进行分析，并筛选铁代谢基因。分离株形成了两个簇，186dz（1A）和Ye8（1B/O:8）为单株。在红细胞浓缩物中培养28天后，细菌计数范围为1.98×10⁵至1.2×10⁹ CFU/mL，其中Ye8（1B/O:8）生长最高，一株4/O:3分离株生长最低。所有分离株在NHS中存活15 - 30分钟，但28s分离株在60分钟时未存活。两株分离株的血清敏感性增加，三株降低，五株保持不变。分离株含有27 - 42个铁代谢基因，具有多个等位基因变体。铁代谢基因的含量或变体可能会影响其在红细胞中的生长。
