Lee Geon Joon, Attri Pankaj, Choi Eun Ha, Kwon Young-Wan, Krasnikov Ilya, Seteikin Alexey
J Nanosci Nanotechnol. 2014 Jan;14(1):221-49. doi: 10.1166/jnn.2014.9105.
In this review, the optical and structural properties of biomaterials are discussed. First, we demonstrate the optical and structural properties of natural and plasma-treated DNA, using UV-visible absorption, circular dichroism (CD), and Raman spectroscopy. Fluorescence and lasing action in the dye-doped DNA-surfactant complex are also explained. Additionally, nanomaterial-based DNA detection and DNA-templated nanomaterial growth are described. Next, we discuss protein folding studies utilizing fluorescence, CD, and nuclear magnetic resonance (NMR) spectroscopy. From the CD spectra of alpha-chymotrypsin (CT), we estimate the composition of a-helices and the beta-sheets, and random coils in the CT. 1H NMR spectroscopy is used to investigate the thermal effect on the refolding of CT in the presence of an ionic liquid. Finally, we explain the numerical simulation method used for studying the optical properties of biomaterials. Applications of the Monte-Carlo method in photodynamic therapy, skin tissue optics, and bioimaging are described.
在本综述中，我们讨论了生物材料的光学和结构特性。首先，我们利用紫外可见吸收光谱、圆二色性（CD）光谱和拉曼光谱，展示了天然DNA和经等离子体处理的DNA的光学和结构特性。同时也解释了染料掺杂的DNA-表面活性剂复合物中的荧光和激光作用。此外，还描述了基于纳米材料的DNA检测以及以DNA为模板的纳米材料生长。接下来，我们讨论利用荧光光谱、CD光谱和核磁共振（NMR）光谱进行的蛋白质折叠研究。从α-胰凝乳蛋白酶（CT）的CD光谱中，我们估算了CT中α-螺旋、β-折叠和无规卷曲的组成。利用1H NMR光谱研究了离子液体存在下热对CT重折叠的影响。最后，我们解释了用于研究生物材料光学特性的数值模拟方法。描述了蒙特卡罗方法在光动力疗法、皮肤组织光学和生物成像中的应用。
