Schmidt R, Cathelineau C, Cavey M T, Dionisius V, Michel S, Shroot B, Reichert U
Centre International de Recherches Dermatologiques, Valbonne, France.
J Cell Physiol. 1989 Aug;140(2):281-7. doi: 10.1002/jcp.1041400213.
Sodium butyrate affects cell differentiation in confluent epidermal keratinocyte cultures by considerably increasing the spontaneous formation of cross-linked envelopes in normal human keratinocytes (NHK). It also favors the development of envelope competence in the Simian virus-40 (SV-40)-transformed human foreskin keratinocyte line SV-K14. It completely abolishes the inhibitory effect of serum and retinoic acid on the expression of plasma membrane-associated transglutaminase. However, other markers of epidermal differentiation that are also under the control of retinoids such as keratins or the enzyme cholesterol sulfotransferase are not affected by butyrate. The level of the cellular retinoic acid binding protein (CRABP) is considerably increased in its presence. Butyrate does not interfere with the binding of retinoids to their cellular binding proteins. Our observations suggest that sodium butyrate stimulates cornified envelope formation via the induction of the plasma membrane-associated transglutaminase required for cornified envelope synthesis and, additionally, by abolishing the inhibitory effect of retinoids on the expression of this enzyme.
丁酸钠通过显著增加正常人角质形成细胞（NHK）中交联包膜的自发形成，影响汇合的表皮角质形成细胞培养物中的细胞分化。它还有利于猿猴病毒40（SV - 40）转化的人包皮角质形成细胞系SV - K14中包膜能力的发展。它完全消除了血清和视黄酸对质膜相关转谷氨酰胺酶表达的抑制作用。然而，其他受类视黄醇调控的表皮分化标志物，如角蛋白或胆固醇硫酸转移酶，不受丁酸盐的影响。在其存在下，细胞视黄酸结合蛋白（CRABP）的水平显著增加。丁酸盐不干扰类视黄醇与其细胞结合蛋白的结合。我们的观察结果表明，丁酸钠通过诱导角质化包膜合成所需的质膜相关转谷氨酰胺酶，以及消除类视黄醇对该酶表达的抑制作用，刺激角质化包膜的形成。
