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基于热稳定O抗原的嗜麦芽窄食单胞菌(嗜麦芽假单胞菌)血清学分型

Serological classification of Xanthomonas maltophilia (Pseudomonas maltophilia) based on heat-stable O antigens.

作者信息

Schable B, Rhoden D L, Hugh R, Weaver R E, Khardori N, Smith P B, Bodey G P, Anderson R L

机构信息

Hospital Infections Program, Centers for Disease Control, Atlanta, Georgia 30333.

出版信息

J Clin Microbiol. 1989 May;27(5):1011-4. doi: 10.1128/jcm.27.5.1011-1014.1989.

Abstract

Twenty-six serotypes of Xanthomonas maltophilia were defined by using 15 antisera described by Hugh and Ryschenkow (R. Hugh and E. Ryschenkow, J. Gen. Microbiol. 26:123-132, 1961) and 11 new antisera. The antisera were prepared by immunizing rabbits with bacterial strains heated at 100 degrees C for 2 h. Twelve antisera required adsorptions with cross-reacting heterologous immunizing strains. We tested 275 clinical and environmental strains of X. maltophilia with 26 antisera by the slide agglutination technique. A total of 259 (94.2%) strains were typeable, with 137 (49.8%) strains agglutinating in three antisera.

摘要

利用休和里斯琴科夫(R. 休和E. 里斯琴科夫,《普通微生物学杂志》26:123 - 132, 1961年)描述的15种抗血清以及11种新抗血清,确定了26个嗜麦芽窄食单胞菌血清型。这些抗血清是通过用在100℃加热2小时的细菌菌株免疫兔子制备的。12种抗血清需要用交叉反应的异源免疫菌株进行吸附处理。我们通过玻片凝集技术用26种抗血清检测了275株嗜麦芽窄食单胞菌临床和环境菌株。总共259株(94.2%)菌株可分型,其中137株(49.8%)在三种抗血清中发生凝集。

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