Chatelut M, Dournes J L, Chabanon G, Marty N
Laboratoire de Microbiologie, Centre Hospitalier Universitaire Rangueil, Toulouse, France.
J Clin Microbiol. 1995 Apr;33(4):912-4. doi: 10.1128/jcm.33.4.912-914.1995.
We used two PCR methods for epidemiological typing of Stenotrophomonas (Xanthomonas) maltophilia with either arbitrary primers (random amplified polymorphic DNA) or enterobacterial repetitive intergenic consensus sequences as primers (ERIC-PCR). The analysis was performed with 38 isolates of S. maltophilia, comprising 9 nosocomial isolates from a burn unit, 20 other clinical isolates epidemiologically unrelated, and 9 isolates from one cystic fibrosis patient. Both methods indicated that all of the nosocomial episodes were independent. In contrast, the nine isolates from the cystic fibrosis patient were assigned to very closely related profiles, especially by ERIC-PCR. We conclude that random amplified polymorphic DNA and ERIC-PCR have comparable reproducible and discriminatory powers for epidemiological typing of S. maltophilia, but ERIC-PCR profiles can be more easily evaluated.
我们使用了两种聚合酶链反应（PCR）方法对嗜麦芽窄食单胞菌（黄单胞菌属）进行流行病学分型，一种是以任意引物（随机扩增多态性DNA）进行，另一种是以肠杆菌重复基因间共有序列作为引物（ERIC-PCR）。对38株嗜麦芽窄食单胞菌进行了分析，其中包括9株来自烧伤病房的医院感染分离株、20株其他在流行病学上无关联的临床分离株以及9株来自一名囊性纤维化患者的分离株。两种方法均表明所有医院感染事件都是独立的。相比之下，来自囊性纤维化患者的9株分离株被归为非常密切相关的图谱类型，尤其是通过ERIC-PCR方法。我们得出结论，随机扩增多态性DNA和ERIC-PCR在嗜麦芽窄食单胞菌的流行病学分型方面具有相当的可重复性和鉴别能力，但ERIC-PCR图谱更容易评估。
