Lee Gyu-Cheol, Kim Min-Jeong, Nam Sehee, Lee Chan Hee
Water Analysis and Research Center, K-water, 200 Sintanjin-ro, Daedeok-gu, Daejeon, 306-711, Korea.
Microbiol Immunol. 2014 Jun;58(6):342-51. doi: 10.1111/1348-0421.12151.
Several bodies of surface water in Korea were surveyed for the presence of hepatitis A virus (HAV) between 2007 and 2010. Of 265 surface water samples, 9 (3.4%) were HAV-positive. HAVs were mainly detected in the summer (3/62, 4.8%) and spring (4/96, 4.2%) seasons. Comparing different water sources, the highest prevalence (6.6%) of positive samples was seen in lake water, four HAV-positive samples being from lakes. Comparing prevalence rates across the four representative Korean basin systems, no HAVs were found in the Han or Nakdong river basins. The highest HAV prevalence was found in samples from the Yeongsan river and other basins (6.3%); the Geum/Seom river was also found to have a high HAV prevalence (5.7%). HAVs from the nine positive samples were then sequenced and analyzed phylogenetically. Two of the HAVs belong to genotype IA and fall within the same cluster as HAVs 6-3(ASAN4) (EU049548), KANSAN-PS1 (EU049554), and ASAN-KM (EU049563), which were collected from the stools of patients with gastroenteritis in Korea. The seven other HAV nucleotide sequences belong to the genotype IB cluster. This is the first nationwide surveillance of HAV in major Korean water sources.
2007年至2010年间，对韩国的几处地表水进行了甲型肝炎病毒（HAV）检测。在265份地表水样本中，9份（3.4%）HAV呈阳性。HAV主要在夏季（3/62，4.8%）和春季（4/96，4.2%）被检测到。比较不同水源，湖水样本中阳性率最高（6.6%），有4份HAV阳性样本来自湖泊。比较韩国四个代表性流域系统的患病率，汉江或洛东江流域未发现HAV。HAV患病率最高的是荣山江和其他流域的样本（6.3%）；锦江/西海水系也被发现有较高的HAV患病率（5.7%）。然后对9份阳性样本中的HAV进行测序并进行系统发育分析。其中两份HAV属于IA基因型，与从韩国肠胃炎患者粪便中收集的HAV 6-3（牙山4）（EU049548）、KANSAN-PS1（EU049554）和牙山-KM（EU049563）属于同一簇。其他7个HAV核苷酸序列属于IB基因型簇。这是韩国主要水源中首次进行的全国性HAV监测。
