Shiba Kota, Tagaya Motohiro, Hanagata Nobutaka
World Premier International (WPI) Research Center, International Center for Materials Nanoarchitectonics (MANA), National Institute for Materials Science (NIMS) , 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan.
ACS Appl Mater Interfaces. 2014 May 14;6(9):6825-34. doi: 10.1021/am500636d. Epub 2014 Apr 25.
Luminescent titania-fluorescein (FS) hybrid nanoparticles (NPs) were successfully synthesized by a sol-gel reaction of titanium alkoxide in the presence of octadecylamine using a fluidic reactor with a Y-type channel. The molar ratio of FS/Ti ratio was varied in the range from 1/1000 to 1/100 in order to obtain the hybrid NPs with the different luminescent behavior. The shape of the NPs is spherical and their sizes are 400 nm which is almost the same irrespective of the FS content, suggesting the different FS molecular states in one NP. We also demonstrated that the hybrid NPs exhibited a characteristic luminescence; the NPs with the higher and lower FS contents exhibited an enhanced luminescence in PBS and air, respectively, indicating that the FS states responded to the molecular environment. Through cytocompatible experiments using the NPs, it turned out that they had a high compatibility for fibroblasts. Therefore, the preparation of a series of the luminescent NPs with a tunable luminescence property was achieved. The results will lead to a guideline to determine a proper combination between material composition and an environment where they are used, being useful for biomedical applications.
通过在十八胺存在下，使用具有Y型通道的流体反应器使钛醇盐进行溶胶 - 凝胶反应，成功合成了发光二氧化钛 - 荧光素（FS）杂化纳米颗粒（NPs）。为了获得具有不同发光行为的杂化纳米颗粒，FS/Ti的摩尔比在1/1000至1/100的范围内变化。纳米颗粒的形状为球形，其尺寸为400nm，与FS含量无关，几乎相同，这表明一个纳米颗粒中FS分子状态不同。我们还证明了杂化纳米颗粒表现出特征性发光；FS含量较高和较低的纳米颗粒分别在PBS和空气中表现出发光增强，表明FS状态对分子环境有响应。通过使用纳米颗粒的细胞相容性实验，结果表明它们对成纤维细胞具有高相容性。因此，实现了一系列具有可调发光性质的发光纳米颗粒的制备。这些结果将为确定材料组成与使用环境之间的适当组合提供指导，对生物医学应用有用。
