Huang Xianfeng, Tang Long, Wang Fan, Song Guoqiang
School of Pharmaceutical Engineering & Life Science, Changzhou University, Changzhou 213164, PR China.
School of Pharmaceutical Engineering & Life Science, Changzhou University, Changzhou 213164, PR China.
Immunobiology. 2014 Jul;219(7):565-71. doi: 10.1016/j.imbio.2014.03.005. Epub 2014 Mar 20.
Astragaloside IV is the chief ingredient of Radix Astragali, which has been used in the Traditional Chinese Medicine as a major component of many polyherbal formulations for the repair and regeneration of injured organ and tissues. We tested the anti-asthmatic effects of AST IV and the possible mechanisms. BALB/c mice that were sensitized and challenged to ovalbumin (OVA) were treated with AST IV (40mg/kg and 20mg/kg) 1h before they were challenged with OVA. Our study demonstrated that AST IV inhibited OVA-induced increases in eosinophil count; interleukin (IL)-4 level were recovered in bronchoalveolar lavage fluid increased IFN-γ and IL-10 levels in bronchoalveolar lavage fluid. Histological studies demonstrated that AST IV substantially inhibited OVA-induced eosinophilia in lung tissue. Flow cytometry studies demonstrated that AST IV substantially increased CD4(+)CD25(+)Foxp3 T cells (Treg). Furthermore quantitative real-time (qPCR) studies demonstrated that AST IV substantially enhanced Foxp3 mRNA expression in lung tissue. These findings suggest that AST IV may effectively ameliorate the progression of airway inflammation and could be used as a therapy for patients with allergic inflammation.
黄芪甲苷是黄芪的主要成分，在传统中药中，它是许多用于受损器官和组织修复与再生的复方制剂的主要成分。我们测试了黄芪甲苷的抗哮喘作用及其可能的机制。用卵清蛋白（OVA）致敏并激发的BALB/c小鼠在接受OVA激发前1小时用黄芪甲苷（40mg/kg和20mg/kg）进行处理。我们的研究表明，黄芪甲苷抑制了OVA诱导的嗜酸性粒细胞计数增加；支气管肺泡灌洗液中升高的白细胞介素（IL）-4水平恢复，支气管肺泡灌洗液中干扰素-γ和IL-10水平升高。组织学研究表明，黄芪甲苷显著抑制了OVA诱导的肺组织嗜酸性粒细胞增多。流式细胞术研究表明，黄芪甲苷显著增加了CD4(+)CD25(+)Foxp3 T细胞（调节性T细胞）。此外，定量实时（qPCR）研究表明，黄芪甲苷显著增强了肺组织中Foxp3 mRNA的表达。这些发现表明，黄芪甲苷可能有效改善气道炎症的进展，并可用于治疗过敏性炎症患者。
