Li Meiliang, Zhang Tuo, Yang Haixia, Zhao Guanghua, Xu Chuanshan
CAU & ACC Joint-Laboratory of Space Food, College of Food Science and Nutritional Engineering, China Agricultural University, Key Laboratory of Functional Dairy, Ministry of Education, Beijing 100083, China; College of Food Science, Sichuan Agricultural University, Yaan, 625014 Sichuan, China.
CAU & ACC Joint-Laboratory of Space Food, College of Food Science and Nutritional Engineering, China Agricultural University, Key Laboratory of Functional Dairy, Ministry of Education, Beijing 100083, China.
Bone. 2014 Jul;64:115-23. doi: 10.1016/j.bone.2014.04.006. Epub 2014 Apr 13.
The consumption of milk is declining in industrialized countries, leading to inadequate calcium intake. Therefore, it is important to explore a new class of Ca-enriched nutrient for the fortification of food. In this work, we prepared a novel class of soluble and edible Ca-protein complexes where approximately 140 calcium ions were encapsulated within a phytoferritin nanocage. As an alternative to other organic and/or inorganic carriers, protein nanocages were found to provide a unique vehicle of biological origin for the intracellular delivery of calcium ions for supplementation. Such encapsulation can protect calcium ions within protein cages against dietary factors such as tannic acid (TA), oxalic acid (OA), and other divalent metal ions in foodstuffs. We demonstrated that the calcium-containing ferritin composites can be absorbed by Caco-2 cells through a process where a TfR1 receptor is involved, whereas the uptake of free calcium ions has been known to be associated with another receptor, DMT1, indicating that the calcium ions encapsulated in supramolecular protein cages can be internalized by the Caco-2 cells through a different pathway from its free analogs for calcium supplementation.
在工业化国家，牛奶的消费量正在下降，导致钙摄入量不足。因此，探索一类新的富含钙的营养素用于食品强化很重要。在这项工作中，我们制备了一类新型的可溶且可食用的钙 - 蛋白质复合物，其中约140个钙离子被包裹在植物铁蛋白纳米笼中。作为其他有机和/或无机载体的替代品，蛋白质纳米笼被发现可为细胞内补充钙离子提供一种独特的生物源载体。这种包封可以保护蛋白质笼内的钙离子免受诸如单宁酸（TA）、草酸（OA）和食品中其他二价金属离子等膳食因素的影响。我们证明含钙质的铁蛋白复合物可通过涉及转铁蛋白受体1（TfR1）的过程被Caco - 2细胞吸收，而游离钙离子的摄取已知与另一种受体二价金属离子转运体1（DMT1）相关，这表明包裹在超分子蛋白质笼中的钙离子可以通过与其游离类似物不同的途径被Caco - 2细胞内化以进行钙补充。
