Cai Xixi, Lin Jiaping, Wang Shaoyun
The Key Lab of Analysis and Detection Technology for Food Safety of the MOE, College of Chemistry, Fuzhou University, Fuzhou 350108, China.
College of Biological Science and Technology, Fuzhou University, Fuzhou 350108, China.
Mar Drugs. 2016 Dec 27;15(1):3. doi: 10.3390/md15010003.
Peptide-calcium can probably be a suitable supplement to improve calcium absorption in the human body. In this study, a specific peptide Phe-Tyr (FY) with calcium-binding capacity was purified from sp. protein hydrolysates through gel filtration chromatography and reversed phase HPLC. The calcium-binding capacity of FY reached 128.77 ± 2.57 μg/mg. Results of ultraviolet spectroscopy, fluorescence spectroscopy, and infrared spectroscopy showed that carboxyl groups, amino groups, and amido groups were the major chelating sites. FY-Ca exhibited excellent thermal stability and solubility, which were beneficial to be absorbed and transported in the basic intestinal tract of the human body. Moreover, the calcium bioavailability in Caco-2 cells showed that FY-Ca could enhance calcium uptake efficiency by more than three times when compared with CaCl₂, and protect calcium ions against dietary inhibitors, such as tannic acid, oxalate, phytate, and Zn. Our findings further the progress of algae-based peptide-calcium, suggesting that FY-Ca has the potential to be developed as functionally nutraceutical additives.
肽钙可能是一种合适的补充剂，可提高人体对钙的吸收。在本研究中，通过凝胶过滤色谱和反相高效液相色谱从 种蛋白质水解物中纯化出一种具有钙结合能力的特定肽苯丙氨酸 - 酪氨酸（FY）。FY的钙结合能力达到128.77±2.57μg/mg。紫外光谱、荧光光谱和红外光谱结果表明，羧基、氨基和酰胺基是主要的螯合位点。FY - Ca表现出优异的热稳定性和溶解性，有利于在人体肠道碱性环境中被吸收和运输。此外，在Caco - 2细胞中的钙生物利用度表明，与CaCl₂相比，FY - Ca可将钙摄取效率提高三倍以上，并保护钙离子免受膳食抑制剂如单宁酸、草酸盐、植酸盐和锌的影响。我们的研究结果推动了基于藻类的肽钙的进展，表明FY - Ca有潜力被开发为功能性营养添加剂。
