Sugino Ilene K, Sun Qian, Cheewatrakoolpong Noounanong, Malcuit Christopher, Zarbin Marco A
Institute of Ophthalmology and Visual Science, Rutgers New Jersey Medical School, Rutgers University, Newark, N.J., USA.
Dev Ophthalmol. 2014;53:133-42. doi: 10.1159/000358531. Epub 2014 Apr 10.
Suspensions of human embryonic stem cell-derived retinal pigment epithelium (hES-RPE) and human fetal RPE resurface aged and age-related macular degeneration (AMD) Bruch's membrane to a limited degree at day 21 in organ culture. Survival and differentiation of hES-RPE and human fetal RPE on aged or AMD Bruch's membrane are enhanced greatly (200%) if a biologically synthesized extracellular matrix (bovine corneal endothelial cell extracellular matrix) is laid down on Bruch's membrane prior to transplantation. Transplanted RPE survival is enhanced even more (400-1,000%) if Bruch's membrane is treated with bovine corneal endothelial cell-conditioned medium during organ culture of hES-RPE or fetal RPE on aged or AMD Bruch's membrane. Future efforts are focused on identifying the bioactive components of bovine corneal endothelial cell-conditioned medium, so that this material can be reconstituted for clinical use as an adjunct to improve RPE transplant survival and differentiation in AMD eyes.
在器官培养的第21天，人胚胎干细胞来源的视网膜色素上皮（hES-RPE）和人胎儿视网膜色素上皮的悬浮液在一定程度上可使衰老及与年龄相关的黄斑变性（AMD）的布鲁赫膜重新表面化。如果在移植前将生物合成的细胞外基质（牛角膜内皮细胞细胞外基质）铺在布鲁赫膜上，hES-RPE和人胎儿视网膜色素上皮在衰老或AMD布鲁赫膜上的存活和分化会大大增强（200%）。如果在hES-RPE或胎儿视网膜色素上皮在衰老或AMD布鲁赫膜上进行器官培养期间，用牛角膜内皮细胞条件培养基处理布鲁赫膜，移植的视网膜色素上皮存活会进一步增强（400 - 1000%）。未来的工作重点是确定牛角膜内皮细胞条件培养基的生物活性成分，以便可以重新配制这种材料用于临床，作为改善AMD眼中视网膜色素上皮移植存活和分化的辅助手段。
