Kittler Sophie, Fischer Samuel, Abdulmawjood Amir, Glünder Gerhard, Klein Günter
Institute of Food Quality and Food Safety, University of Veterinary Medicine Hannover, Hannover, Germany.
Clinic for Poultry, University of Veterinary Medicine Hannover, Hannover, Germany.
PLoS One. 2014 Apr 14;9(4):e94782. doi: 10.1371/journal.pone.0094782. eCollection 2014.
The pathogens Campylobacter jejuni and Campylobacter coli are commensals in the poultry intestine and campylobacteriosis is one of the most frequent foodborne diseases in developed and developing countries. Phages were identified to be effective in reducing intestinal Campylobacter load and this was evaluated, in the first field trials which were recently carried out. The aim of this study was to further investigate Campylobacter population dynamics during phage application on a commercial broiler farm. This study determines the superiority in colonisation of a Campylobacter type found in a field trial that was susceptible to phages in in vitro tests. The colonisation factors, i.e. motility and gamma glutamyl transferase activity, were increased in this type. The clustering in phylogenetic comparisons of MALDI-TOF spectra did not match the ST, biochemical phenotype and phage susceptibility. Occurrence of Campylobacter jejuni strains and phage susceptibility types with different colonisation potential seem to play a very important role in the success of phage therapy in commercial broiler houses. Thus, mechanisms of both, phage susceptibility and Campylobacter colonisation should be further investigated and considered when composing phage cocktails.
空肠弯曲菌和结肠弯曲菌这两种病原体是家禽肠道中的共生菌，弯曲菌病是发达国家和发展中国家最常见的食源性疾病之一。噬菌体被证实可有效降低肠道中弯曲菌的数量，最近进行的首批田间试验对此进行了评估。本研究的目的是进一步调查在商业肉鸡场应用噬菌体期间弯曲菌的种群动态。本研究确定了在体外试验中对噬菌体敏感的一种弯曲菌类型在田间试验中的定殖优势。该类型的定殖因子，即运动性和γ-谷氨酰转移酶活性有所增加。基质辅助激光解吸电离飞行时间质谱（MALDI-TOF）光谱的系统发育比较中的聚类与序列类型（ST）、生化表型和噬菌体敏感性不匹配。具有不同定殖潜力的空肠弯曲菌菌株和噬菌体敏感性类型的出现似乎在商业肉鸡舍噬菌体疗法的成功中起着非常重要的作用。因此，在配制噬菌体混合物时，应进一步研究和考虑噬菌体敏感性和弯曲菌定殖这两种机制。
